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Anti-Mouse SEMA4A Rabbit Recombinant Antibody (98513-2-RR)

Anti-Mouse SEMA4A Rabbit Recombinant Antibody

SEMA4A Uni-rAb^® Recombinant Antibody for FC

Cat No. 98513-2-RR

Clone No. 250325B12

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Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Synonyms

Sema B, Semab, Semaphorin-4A, Semaphorin-B, SemB

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

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FC experiment of mouse splenocytes using 98513-2-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Mouse CD3. Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of mouse splenocytes using 98513-2-RR
1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293T using 98513-2-RR
1x10^6 HEK-293T cells or SEMA4A transfected HEK-293T cells were surface stained with 0.25 ug Anti-Mouse Sema4a Rabbit RecAb (98513-2-RR, Clone: 250325B12) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Transfected HEK-293T using 98513-2-RR
1x10^6 HEK-293T cells (left) or SEMA4A transfected HEK-293T cells (right) were surface stained with 0.25 ug Anti-Mouse SEMA4A Rabbit RecAb (98513-2-RR, Clone: 250325B12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 98513-2-RR
Biolayer interferometry (BLl) kinetic assays of 98513-2-RR against Mouse SEMA4A were performed. The affinity constant is below 1 pM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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What's the best dilution for SEMA4A in ?

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive FC detected in

mouse splenocytes, Transfected HEK-293T cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98513-2-RR targets SEMA4A in FC applications and shows reactivity with mouse samples.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg2680 Product name: Recombinant Mouse SEMA4A protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 33-682 aa of NM_001163489.1 Sequence: TGGQGPMPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSDDGNTLYVGAREAVLALNIQNPGIPRLKNMIPWPASERKKTECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSLLLPILIDKVMDGKGQSPFDPVHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHADASFVAAIPSTQVVYFFFEETASEFDFFEELYISRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVSRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVSPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGTPLLVKSGVEYTRLAVESARGLDGSSHVVMYLGTSTGSLHKAVVPQDSSAYLVEEIQLSPDSEPVRNLQLAPAQGAVFAGFSGGIWRVPRANCSVYESCVDCVLARDPHCAWDPESRLCSLLSGSTKPWKQDMERGNPEWVCTRGPMARSPRRQSPPQLIKEVLTVPNSILELPCPHLSALASYHWSHGRAKISEASATVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPH Predict reactive species
Full Name	sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
Calculated Molecular Weight	83 kDa
GenBank Accession Number	NM_001163489.1
Gene Symbol	Sema4a
Gene ID (NCBI)	20351
RRID	AB_3746143
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q62178
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.