Tested Applications
| Positive FC detected in | mouse peripheral blood platelets |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
98516-2-RR targets JAM-A/CD321 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg3117 Product name: Recombinant Mouse JAM-A/Junctional Adhesion Molecule A protein (rFc Tag) (HPLC verified) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 27-238 aa of NM_172647.2 Sequence: KGSVYTAQSDVQVPENESIKLTCTYSGFSSPRVEWKFVQGSTTALVCYNSQITAPYADRVTFSSSGITFSSVTRKDNGEYTCMVSEEGGQNYGEVSIHLTVLVPPSKPTISVPSSVTIGNRAVLTCSEHDGSPPSEYSWFKDGISMLTADAKKTRAFMNSSFTIDPKSGDLIFDPVTAFDSGEYYCQAQNGYGTAMRSEAAHMDAVELNVGG Predict reactive species |
| Full Name | F11 receptor |
| Calculated Molecular Weight | 32 kDa |
| GenBank Accession Number | NM_172647.2 |
| Gene Symbol | F11r |
| Gene ID (NCBI) | 16456 |
| RRID | AB_3746145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O88792 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2 - 8°C. Stable for one year after shipment. |
Background Information
Junctional adhesion molecule A (JAM-A), also known as CD321 or F11 receptor (F11R), is a transmembrane protein that belongs to the immunoglobulin superfamily of cell adhesion molecules. JAM-A is present in epithelial cells, endothelial cells, leukocytes, and blood platelets. JAM-A participates in regulating various biological processes, as diverse as paracellular permeability, tight junction formation and maintenance, leukocyte transendothelial migration, epithelial-to-mesenchymal transition, angiogenesis, reovirus binding, and platelet activation.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for JAM-A/CD321 antibody 98516-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
