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Anti-Human IL-10 Rabbit Recombinant Antibody (98525-2-RR)

Anti-Human IL-10 Rabbit Recombinant Antibody

IL-10 Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. 98525-2-RR

Clone No.250059F11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

IL-10,IL10, CSIF, IL 10, IL10, IL10A

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

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Filter: AllFC (Intra)

FC experiment of human PBMCs using 98525-2-RR
1x10^6 untreated or anti-CD3/CD28, IL-2 and IL-4 treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-10 Rabbit RecAb (98525-2-RR, Clone: 250059F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Human CD4 Rabbit Recombinant Antibody (CL488-98042, Clone: 240427E12). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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1x10^6 untreated or anti-CD3/CD28, IL-2 and IL-4 treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-10 Rabbit RecAb (98525-2-RR, Clone: 250059F11) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 488 Anti-Human CD4 Rabbit Recombinant Antibody (CL488-98042, Clone: 240427E12). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive FC (Intra) detected in

anti-CD3/CD28, IL-2 and IL-4 treated human PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98525-2-RR targets IL-10 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg3296 Product name: Recombinant Human IL-10 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 19-178 aa of NM_000572.3 Sequence: SPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN Predict reactive species
Full Name	interleukin 10
Calculated Molecular Weight	21 kDa
GenBank Accession Number	NM_000572.3
Gene Symbol	IL-10
Gene ID (NCBI)	3586
RRID	AB_3746153
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P22301
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.