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Anti-Human CD19 Rabbit Recombinant Antibody (98560-3-RR)

Anti-Human CD19 Rabbit Recombinant Antibody

CD19 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98560-3-RR

Clone No.253337A1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, cynomolgus monkey

Applications

Synonyms

B lymphocyte antigen CD19, B4, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, CD19 molecule

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllFC

FC experiment of Human PBMCs using 98560-3-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 Rabbit RecAb (98560-3-RR, Clone: 253337A1) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with FcZero-rAb® APC Anti-Human CD3 Rabbit Recombinant Antibody (APC-FcA98319-2, Clone: SP34). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Human PBMCs using 98560-3-RR
1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD19 Rabbit RecAb (98560-3-RR, Clone: 253337A1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Cyno PBMCs using 98560-3-RR
1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD19 Rabbit RecAb (98560-3-RR, Clone: 253337A1) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with FcZero-rAb® APC Anti-Human CD3 Rabbit Recombinant Antibody (APC-FcA98319-2, Clone: SP34). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of Cyno PBMCs using 98560-3-RR
1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD19 Rabbit RecAb (98560-3-RR, Clone: 253337A1) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive FC detected in

Human PBMCs, Cynomolgus PBMCs

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98560-3-RR targets CD19 in FC applications and shows reactivity with human, cynomolgus monkey samples.

Tested Reactivity	human, cynomolgus monkey
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	unknow Predict reactive species
Full Name	CD19 molecule
Gene Symbol	CD19
Gene ID (NCBI)	930
ENSEMBL Gene ID	ENSG00000177455
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P15391
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

CD19 is a 95 kDa type I transmembrane glycoprotein belonging to the immunoglobulin superfamily (PMID: 2472450). It is expressed by B cells and follicular dendritic cells. CD19 is up-regulated at the step of B-lineage commitment during the differentiation of the hematopoietic stem cell, it remains on during subsequent stages of differentiation until finally down-regulated during terminal differentiation into plasma cells (PMID: 8528044). CD19 is involved in B cell development, activation and differentiation. It is the dominant component for the signaling complex on B cells that includes CD21 (CR2), CD81 (TAPA-1) and CD225 and acts as a critical co-receptor for BCR signal transduction (PMID: 23210908).