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Anti-Human Mesothelin Rabbit Recombinant Antibody (98776-3-RR)

Anti-Human Mesothelin Rabbit Recombinant Antibody

Mesothelin Uni-rAb^® Recombinant Antibody for IHC, FC

Cat No. 98776-3-RR

Clone No.252795C4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

MSLN, CAK1, CAK1 antigen, Megakaryocyte-potentiating factor, Mesothelin, cleaved form

Formulation: PBS, Azide

PBS, Azide

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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IHC staining of human ovary cancer using 98776-3-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 98776-3-RR (Mesothelin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 98776-3-RR
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 98776-3-RR (Mesothelin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of HeLa using 98776-3-RR
1x10^6 HeLa cells (left) or PC-3 cells (right) were surface stained with 0.25 ug Anti-Human Mesothelin Rabbit RecAb (98776-3-RR, Clone: 252795C4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive IHC detected in	human ovary cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive FC detected in	HeLa cells

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98776-3-RR targets Mesothelin in IHC, FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	DNA Predict reactive species
Full Name	mesothelin
Calculated Molecular Weight	69KD
GenBank Accession Number	NM_005823
Gene Symbol	MSLN
Gene ID (NCBI)	10232
RRID	AB_3746342
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13421
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Mesothelin (MSLN) is a 40-kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71 kDa precursor protein that is processed to a 40-kDa membrane-bound protein termed mesothelin and a 31-kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).