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Anti-Human DSG2 Rabbit Recombinant Antibody (98863-1-RR)

Anti-Human DSG2 Rabbit Recombinant Antibody

DSG2 Uni-rAb^® Recombinant Antibody for FC

Cat No. 98863-1-RR

Clone No.251405F11

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Synonyms

ARVC10, ARVD10, Cadherin family member 5, CDHF5, Desmoglein 2

Formulation: PBS, Azide

PBS Only

Conjugate: Unconjugated

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Filter: AllFC

FC experiment of HeLa using 98863-1-RR
1x10^6 HeLa cells were surface stained with 0.25 ug Anti-Human DSG2 Rabbit RecAb (98863-1-RR, Clone: 251405F11) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of CHO using 98863-1-RR
1x10^6 CHO cells or DSG2 transfected CHO cells were surface stained with 0.25 ug Anti-Human DSG2 Rabbit RecAb (98863-1-RR, Clone: 251405F11) and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
FC experiment of CHO using 98863-1-RR
1x10^6 CHO cells or DSG2-transfected CHO cells were surface stained with 0.25 ug Anti-Human DSG2 Rabbit RecAb (98863-1-RR, Clone: 251405F11) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and CoraLite647-conjugated F(ab')2 Fragment Donkey Anti-Rabbit IgG (H+L) (SA00014-7). Cells were not fixed.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive FC detected in

HeLa cells, Transfected CHO cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC)	FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98863-1-RR targets DSG2 in FC applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Eg6509 Product name: Recombinant Human DSG2 protein (rFc Tag) Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 50-609 aa of NM_001943.4 Sequence: AWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITEPPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVFVGSVEELSAAHTLVMKINATDADEPNTLNSKISYRIVSLEPAYPPVFYLNKDTGEIYTTSVTLDREEHSSYTLTVEARDGNGEVTDKPVKQAQVQIRILDVNDNIPVVENKVLEGMVEENQVNVEVTRIKVFDADEIGSDNWLANFTFASGNEGGYFHIETDAQTNEGIVTLIKEVDYEEMKNLDFSVIVANKAAFHKSIRSKYKPTPIPIKVKVKNVKEGIHFKSSVISIYVSESMDRSSKGQIIGNFQAFDEDTGLPAHARYVKLEDRDNWISVDSVTSEIKLAKLPDFESRYVQNGTYTVKIVAISEDYPRKTITGTVLINVEDINDNCPTLIEPVQTICHDAEYVNVTAEDLDGHPNSGPFSFSVIDKPPGMAEKWKIARQESTSVLLQQSEKKLGRSEIQFLISDNQGFSCPEKQVLTLTVCECLHGSGCREAQHDSYVG Predict reactive species
Full Name	desmoglein 2
Calculated Molecular Weight	122 kDa
GenBank Accession Number	NM_001943.4
Gene Symbol	DSG2
Gene ID (NCBI)	1829
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q14126
Storage Buffer	PBS with 0.09% sodium azide, pH 7.3.
Storage Conditions	Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Desmosomal cadherins, consisting of four desmogleins (DSG1-4) and three desmocollins (DSC1-3) in humans, mediate adhesion through calcium-dependent homophilic/heterophilic interactions. DSG2 is a single-pass transmembrane glycoprotein that is widely expressed in epithelial and non-epithelial tissues, such as the intestine, epidermis, testis, and heart (PMID:21715983). Defects in DSG2 are the cause of familial arrhythmogenic right ventricular dysplasia type 10 (ARVD10), and genetic variations in DSG2 are the cause of susceptibility to cardiomyopathy dilated type 1BB (CMD1BB).