Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.2 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-65065 targets CD49b in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rat / IgM, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
NK cells isolated from C57BL/6 mice Predict reactive species |
| Full Name | integrin alpha 2 |
| GenBank Accession Number | BC065139 |
| Gene Symbol | CD49b |
| Gene ID (NCBI) | 16398 |
| RRID | AB_2882967 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Affinity purification |
| UNIPROT ID | Q62469 |
| Storage Buffer | PBS with 0.1% sodium azide and a stabilizer, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
Integrins are a large family of α/β transmembrane receptors playing a key role in cell-cell interactions and cell adhesion to the extracellular matrix (PMID: 30374344). CD49b, also known as integrin alpha-2, is a 150 kDa transmembrane glycoprotein expressed on a wide variety of cell types including platelets, megakaryocytes, NK cells, NKT cells, neutrophils, and long-term activated T cells (PMID: 18802064; 2415516). Integrin alpha-2/beta-1 (also known as VLA-2) is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin (PMID: 2557634). It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and CLG gene expression, force generation and organization of newly synthesized extracellular matrix.
Publications
What published studies show
APC-65065 (Anti-Mouse CD49b/DX5) has 1 indexed citation published in Liver International (IF 6.2, 2022). The study investigated the role of human poliovirus receptor in biliary atresia pathogenesis, specifically how it exacerbates natural killer cell–mediated bile duct injury. The research field encompasses hepatobiliary disease and innate immune cell biology, with flow cytometry as the cited application.
| Species | Application | Title |
|---|---|---|
Liver Int Human poliovirus receptor contributes to biliary atresia pathogenesis by exacerbating natural-killer-cell-mediated bile duct injury |
