Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
APC-FcA65655 targets CD4 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | CD4 antigen |
| GenBank Accession Number | BC039137 |
| Gene Symbol | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_3746453 |
| Conjugate | APC Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 650 nm / 660 nm |
| Excitation Laser | Red Laser (633 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P06332 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for APC CD4 antibody APC-FcA65655 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
APC-FcA65655 is the FcZero-rAb® Anti-Mouse CD4 (RM4-4) Rabbit Recombinant Antibody, with a total of 4 citations. The cited publications appear in Veterinary Microbiology and Cancer Letters. The research fields span veterinary immunology (peptide vaccine-induced protective immune responses in swine) and cancer biology (ovarian cancer proliferation, neutrophil infiltration, and neutrophil extracellular trap formation via the IRAK4-CXCL5 axis). The antibody was applied in flow cytometry for mouse species.
| Species | Application | Title |
|---|---|---|
Vet Microbiol Synthetic peptide vaccines targeting SVA elicit robust protective immune responses in swine. | ||
Cancer Lett Verbascoside inhibits ovarian cancer proliferation via IRAK4-CXCL5 axis-mediated suppression of neutrophil infiltration and neutrophil extracellular trap formation. |
