Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
Biotin-SF98190 targets CD98/SLC3A2 in FC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Type | Rabbit / scFv |
| Class | Recombinant |
| Type | Single-chain variable fragment antibody (scFv) |
| Immunogen |
CatNo: Eg1948 Product name: Recombinant Human CD98/SLC3A2 protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 206-630 aa of NM_002394.5 Sequence: RAPRCRELPAQKWWHTGALYRIGDLQAFQGHGAGNLAGLKGRLDYLSSLKVKGLVLGPIHKNQKDDVAQTDLLQIDPNFGSKEDFDSLLQSAKKKSIRVILDLTPNYRGENSWFSTQVDTVATKVKDALEFWLQAGVDGFQVRDIENLKDASSFLAEWQNITKGFSEDRLLIAGTNSSDLQQILSLLESNKDLLLTSSYLSDSGSTGEHTKSLVTQYLNATGNRWCSWSLSQARLLTSFLPAQLLRLYQLMLFTLPGTPVFSYGDEIGLDAAALPGQPMEAPVMLWDESSFPDIPGAVSANMTVKGQSEDPGSLLSLFRRLSDQRSKERSLLHGDFHAFSAGPGLFSYIRHWDQNERFLVVLNFGDVGLSAGLQASDLPASASLPAKADLLLSTQPGREEGSPLELERLKLEPHEGLLLRFPYAA Predict reactive species |
| Full Name | solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2 |
| Calculated Molecular Weight | 68 kDa |
| GenBank Accession Number | NM_002394.5 |
| Gene Symbol | CD98 |
| Gene ID (NCBI) | 6520 |
| RRID | AB_3747079 |
| Conjugate | Biotin |
| Excitation/Emission Maxima Wavelengths | - |
| Source | Anti-Human CD98/SLC3A2 scFv from clone 241755D6, consisting of VH-GGGGS linker-VL (N- to C-terminus), with a His tag at the C-terminus. |
| Form | Liquid |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 4% sucrose and 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. 20ul sizes contain 0.1% BSA. |
Background Information
CD98 is a 125-kDa type II membrane glycoprotein heterodimer composed of a 40-kDa non-glycosylated light chain (amino-acid transporter, encoded by the SLC7A5) and an 85-kDa glycosylated heavy chain (CD98hc, encoded by the SLC3A2) (PMID: 11507094). CD98hc also interacts with certain integrin b-subunits to regulate cell migration, survival, proliferation, and adhesion/polarity. CD98hc is overexpressed on the cell surface of many cancers and increased CD98hc expression is associated with the development and progression of tumors.(PMID:25084765). Molecular orientation studies have demonstrated that the C-terminal tail of human CD98 (hCD98), which contains a PDZ-binding domain, is extracellular (PMID: 18625289).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin CD98/SLC3A2 antibody Biotin-SF98190 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
