Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| FC | See 2 publications below |
Product Information
FITC-65169 targets CD163 in FC applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Glycoprotein preparation from hairy cell leukemia spleen Predict reactive species |
| Full Name | CD163 molecule |
| Calculated Molecular Weight | 1156 aa, 125 kDa |
| GenBank Accession Number | BC051281 |
| Gene Symbol | CD163 |
| Gene ID (NCBI) | 9332 |
| ENSEMBL Gene ID | ENSG00000177575 |
| RRID | AB_2924121 |
| Conjugate | FITC Plus Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 495 nm / 524 nm |
| Form | Liquid |
| Purification Method | Purified by protein-A affinity chromatography |
| UNIPROT ID | Q86VB7 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FITC Plus CD163 antibody FITC-65169 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Tissue Cell Bromodomain containing 4 inhibition combats gastric precancerous lesions via modulating macrophage polarization | ||
Discov Oncol Hepatoma cell-derived exosomal SNORD52 mediates M2 macrophage polarization by activating the JAK2/STAT6 pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Julie (Verified Customer) (08-26-2025) | Product used at 5uL per 100uL sample containing approximately 4-5 x 10^5 cells. Only shows slight upregulation in IL-10 treated cells, but literature suggests that this is to be expected. Seemed to work better when cells were not PFA fixed.
|
