Tested Applications
| Positive FC detected in | human PBMCs |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 5 ul per 10^6 cells in 100 μl suspension |
| This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-65169 targets CD163 in IF, FC applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Glycoprotein preparation from hairy cell leukemia spleen Predict reactive species |
| Full Name | CD163 molecule |
| Calculated Molecular Weight | 1156 aa, 125 kDa |
| GenBank Accession Number | BC051281 |
| Gene Symbol | CD163 |
| Gene ID (NCBI) | 9332 |
| ENSEMBL Gene ID | ENSG00000177575 |
| RRID | AB_2883212 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Excitation Laser | Blue laser (488 nm) |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q86VB7 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 CD163 antibody CL488-65169 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is Anti-Human CD163 (GHI/61), catalog number CL488-65169, with 3 citations. These appear in Cell Death & Disease, Cell & Molecular Life Sciences, and Oncology Letters. The research fields encompass tumor-associated macrophage biology, exosomal signaling, and cancer progression—specifically pancreatic ductal adenocarcinoma, head and neck squamous cell carcinoma, and non-small cell lung cancer. Applications cited include immunofluorescence and flow cytometry.
| Species | Application | Title |
|---|---|---|
Cell Death Dis Tumor-derived exosomal CCT6A serves as a matchmaker introducing chemokines to tumor-associated macrophages in pancreatic ductal adenocarcinoma | ||
Cell Mol Life Sci Tumor-associated macrophage-derived exosomal miR21-5p promotes tumor angiogenesis by regulating YAP1/HIF-1α axis in head and neck squamous cell carcinoma | ||
Oncol Lett Tumor-associated macrophage-derived exosomes promote EGFR-TKI resistance in non-small cell lung cancer by regulating the AKT, ERK1/2 and STAT3 signaling pathways |
Reviews
What customers say
The single review for CL488-65169 reports only slight staining when used on THP-1 cells (5 µL per 100 µL sample, ~8×10⁵ cells). The reviewer was unsure whether the weak signal was due to the cell type or the conjugation. Despite the staining issue, they rated the product 5 stars, praising the excellent customer service, with multiple staff members helping troubleshoot. They plan to retest the antibody on human PBMC-derived macrophages. Overall, the feedback highlights strong technical support but suggests the staining performance may need optimization for certain cell types.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Julie (Verified Customer) (10-20-2025) | Only showed slight staining, used 5uL per 100uL sample containing approximately 8 x 10^5 cells. Possible because they were THP-1 cells? Or the conjugation affected the antibody. Customer service for this product was excellent though (hence the three stars), with multiple persons involved to help me troubleshoot. I will be trying the product again when I move to human PBMC derived macrophages.
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