|Positive FC detected in||human peripheral blood monocytes|
|This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 μl per 10^6 cells in a 100 µl suspension or 10 μl per 100 µl of whole blood.|
|Sample-dependent, check data in validation data gallery|
PE-65169 targets CD163 in FC applications and shows reactivity with Human samples.
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Glycoprotein preparation from hairy cell leukemia spleen|
|Full Name||CD163 molecule|
|Calculated molecular weight||1156 aa, 125 kDa|
|GenBank accession number||BC051281|
|Gene ID (NCBI)||9332|
|Conjugate||PE Fluorescent Dye|
|Excitation/Emission maxima wavelengths||496 nm, 565 nm / 578 nm|
|Purification Method||The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.|
|Storage Buffer||PBS with 0.09% sodium azide and 0.5% BSA.|
|Storage Conditions||Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.|
CD163, also known as M130, is a membrane glycoprotein which belongs to the scavenger receptor superfamily (PMID: 8370408). It is an acute phase-regulated and signal-inducing macrophage protein expressed exclusively in monocytes and tissue macrophages (PMID: 11196644). CD163 mediates endocytosis of haptoglobin-haemoglobin complexes (PMID: 11196644). The uptake of haptoglobin by macrophages contributes to the recycling of iron and also to the inflammatory response (PMID: 22900885). Soluble CD163 (sCD163), as a result of ectodomain shedding during inflammatory activation of macrophages, circulates in blood and has been suggested as a plasma/serum marker for macrophage activity (PMID: 12570164).