• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Mouse uPAR/CD87 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: mouse

Detection: mouse

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP02130-3

Synonyms

uPAR, PLAUR, PLAUR, CD87, MO3, U PAR

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02130-3, Mouse uPAR/CD87 Recombinant Matched Antibody Pair - PBS only. 85824-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3156. 85824-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

    Sandwich ELISA standard curve of MP02130-3

    Sandwich ELISA standard curve of MP02130-3, Mouse uPAR/CD87 Recombinant Matched Antibody Pair - PBS only. 85824-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3156. 85824-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Urine of six mice was measured. The mouse uPAR/CD87 concentration of detected samples was determined to be 849.5 pg/mL with a range of 395.5 - 1,217.4 pg/mL.

    Sample test of MP02130-3

    Urine of six mice was measured. The mouse uPAR/CD87 concentration of detected samples was determined to be 849.5 pg/mL with a range of 395.5 - 1,217.4 pg/mL.

  • Serum of six mice was measured. The mouse uPAR/CD87 concentration of detected samples was determined to be 10,613.9 pg/mL with a range of 9,282.9 - 11,762.9 pg/mL.

    Sample test of MP02130-3

    Serum of six mice was measured. The mouse uPAR/CD87 concentration of detected samples was determined to be 10,613.9 pg/mL with a range of 9,282.9 - 11,762.9 pg/mL.

  • The mean uPAR/CD87 concentration was determined to be 698.63 ng/mL in mouse lung tissue extract based on a 5.3 mg/mL extract load.

    Sample test of MP02130-3

    The mean uPAR/CD87 concentration was determined to be 698.63 ng/mL in mouse lung tissue extract based on a 5.3 mg/mL extract load.

  • Raw264.7 (5 x 10^5 cells/mL) were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for mouse uPAR/CD87, and measured 22.21 ng/mL.

    Sample test of MP02130-3

    Raw264.7 (5 x 10^5 cells/mL) were cultured in DMEM supplemented with 10% fetal bonive serum. An aliquot of the cell culture supernate was removed, assayed for mouse uPAR/CD87, and measured 22.21 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02130-3 targets uPAR/CD87 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: Mouse uPAR/CD87 Recombinant antibody, PBS Only (Capture/Detector) 85824-2-PBS (242976A5). 100 μg. Concentration 1 mg/ml.

Detection antibody: Mouse uPAR/CD87 Recombinant antibody, PBS Only (Detector) 85824-1-PBS (242976A7). 100 μg. Concentration 1 mg/ml.

Alternative uPAR/CD87 matched antibody pairs: MP02130-1, MP02130-2

Matched pair protein standard: Eg3156

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 85824-2-PBS
Tested Reactivity mouse
Immunogen uPAR/CD87 fusion protein Eg3156
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name plasminogen activator, urokinase receptor
Synonyms CD87, Plaur, uPAR, U-PAR, Urokinase plasminogen activator surface receptor
GenBank accession numberNM_011113.4
Gene symbol Plaur
UniprotP35456
Gene ID (NCBI) 18793
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 85824-1-PBS
Tested Reactivity mouse
Immunogen uPAR/CD87 fusion protein Eg3156
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name plasminogen activator, urokinase receptor
Synonyms CD87, Plaur, uPAR, U-PAR, Urokinase plasminogen activator surface receptor
GenBank accession numberNM_011113.4
Gene symbol Plaur
UniprotP35456
Gene ID (NCBI) 18793
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.