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SUPV3L1 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat no : MP02602-2

Synonyms

ATP-dependent RNA helicase SUPV3L1, mitochondrial, EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3, SUV3 like protein 1

Validation Data Gallery

  • Sandwich ELISA standard curve of MP02602-2, Human SUPV3L1 Recombinant Matched Antibody Pair - PBS only. 86706-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3542. 86706-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

    Sandwich ELISA standard curve of MP02602-2

    Sandwich ELISA standard curve of MP02602-2, Human SUPV3L1 Recombinant Matched Antibody Pair - PBS only. 86706-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3542. 86706-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • The mean SUPV3L1 concentration was determined to be 66.66 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 31.90 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load and 13.66 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

    Sandwich ELISA sample test of MP02602-2

    The mean SUPV3L1 concentration was determined to be 66.66 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 31.90 ng/mL in PC-3 cell extract based on a 1.3 mg/mL extract load and 13.66 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP02602-2 targets SUPV3L1 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: SUPV3L1 Recombinant monoclonal antibody, PBS Only (Capture) 86706-2-PBS (250855G1). 100 μg. Concentration 1 mg/ml.

Detection antibody: SUPV3L1 Recombinant monoclonal antibody, PBS Only (Detector) 86706-3-PBS (250855A8). 100 μg. Concentration 1 mg/ml.

Alternative SUPV3L1 matched antibody pairs: MP02602-1

Matched pair protein standard: Ag3542

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 86706-2-PBS
Tested Reactivity human
Immunogen SUPV3L1 fusion protein Ag3542
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name suppressor of var1, 3-like 1 (S. cerevisiae)
Synonyms
GenBank accession numberBC036112
Gene symbol SUPV3L1
UniprotQ8IYB8
Gene ID (NCBI) 6832
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 86706-3-PBS
Tested Reactivity human
Immunogen SUPV3L1 fusion protein Ag3542
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name suppressor of var1, 3-like 1 (S. cerevisiae)
Synonyms ATP-dependent RNA helicase SUPV3L1, mitochondrial, EC:3.6.4.13, Suppressor of var1 3-like protein 1, SUV3, SUV3 like protein 1
GenBank accession numberBC036112
Gene symbol SUPV3L1
UniprotQ8IYB8
Gene ID (NCBI) 6832
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
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