Tested Applications
| Positive FC detected in | mouse splenocytes |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.1 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
PE-FcA65655 targets CD4 in FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
N/A Predict reactive species |
| Full Name | CD4 antigen |
| GenBank Accession Number | BC039137 |
| Gene Symbol | Cd4 |
| Gene ID (NCBI) | 12504 |
| RRID | AB_3749673 |
| Conjugate | PE Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 496 nm, 565 nm / 578 nm |
| Excitation Laser | Blue Laser (488 nm), Green Laser (532 nm), Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P06332 |
| Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
Background Information
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PE CD4 antibody PE-FcA65655 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PE-FcA65655 is the FcZero-rAb® Anti-Mouse CD4 (RM4-4) Rabbit Recombinant Antibody. It has 2 total citations, published in Advanced Science (IF 14.1) and Journal of Colloid and Interface Science (IF 9.6). Both studies focus on antitumor immunotherapy, specifically involving multifunctional nano immunostimulants for overcoming immunosuppressive microenvironments and nano co-inducers of immunogenic cell death and ferroptosis. The cited application is flow cytometry in mouse models.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Multifunctional Nano Immunostimulant: Overcoming Immunosuppressive Microenvironment for Antitumor Immunotherapy. | ||
J Colloid Interface Sci Nano co-inducer of immunogenic cell death and ferroptosis for anti-tumor immunotherapy |
