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Beta ENaC Polyclonal antibody

Beta ENaC Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14134-1-AP

Synonyms

Beta ENaC, Beta NaCH, ENaCb, ENaCbeta, SCNEB, SCNN1B, β ENaC, βENaC



Tested Applications

Positive WB detected inPC-3 cells, mouse kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14134-1-AP targets Beta ENaC in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity hamster, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Beta ENaC fusion protein Ag5348
Full Name sodium channel, nonvoltage-gated 1, beta
Calculated molecular weight 640 aa, 73 kDa
Observed molecular weight 75-80 kDa
GenBank accession numberBC036352
Gene symbol SCNN1B
Gene ID (NCBI) 6338
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SCNN1B, also named as SCNEB, ENaCB, Beta-NaCH and Beta-ENaC, belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family and SCNN1B subfamily. Sodium permeable non-voltage-sensitive ion channel is inhibited by the diuretic amiloride. SCNN1B mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. It controls the reabsorption of sodium in kidney, colon, lung and sweat glands. SCNN1B plays a role in taste perception.

Protocols

Product Specific Protocols
WB protocol for Beta ENaC antibody 14134-1-APDownload protocol
IHC protocol for Beta ENaC antibody 14134-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Exp Anim

Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathway.

Authors - Bingji Jin
ratWB

Int J Mol Sci

High Salt Diet Affects Renal Sodium Excretion and ERRα Expression.

Authors - Dan Wang
humanWB

Toxins (Basel)

Listeriolysin O Causes ENaC Dysfunction in Human Airway Epithelial Cells.

Authors - Guang Yang
hamsterWB

Exp Cell Res

Calreticulin positively regulates the expression and function of epithelial sodium channel.

Authors - Sugahara Takuya T
  • KD Validated
ratWB

J Neurotrauma

Activity-based training reverses SCI-induced changes in kidney receptor densities and membrane proteins.

Authors - Jason H Gumbel
mouseWB

Front Pharmacol

Melatonin Attenuates Sepsis-Induced Acute Lung Injury Through Improvement of Epithelial Sodium Channel-Mediated Alveolar Fluid Clearance Via Activation of SIRT1/SGK1/Nedd4-2 Signaling Pathway.

Authors - Jing Li