|Positive WB detected in||PC-3 cells, mouse kidney tissue|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14134-1-AP targets Beta ENaC in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||hamster, human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Beta ENaC fusion protein Ag5348|
|Full Name||sodium channel, nonvoltage-gated 1, beta|
|Calculated molecular weight||640 aa, 73 kDa|
|Observed molecular weight||75-80 kDa|
|GenBank accession number||BC036352|
|Gene ID (NCBI)||6338|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
SCNN1B, also named as SCNEB, ENaCB, Beta-NaCH and Beta-ENaC, belongs to the amiloride-sensitive sodium channel (TC 1.A.6) family and SCNN1B subfamily. Sodium permeable non-voltage-sensitive ion channel is inhibited by the diuretic amiloride. SCNN1B mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. It controls the reabsorption of sodium in kidney, colon, lung and sweat glands. SCNN1B plays a role in taste perception.
Oxymatrine attenuates lipopolysaccharide-induced acute lung injury by activating the epithelial sodium channel and suppressing the JNK signaling pathway.
Int J Mol Sci
High Salt Diet Affects Renal Sodium Excretion and ERRα Expression.
Listeriolysin O Causes ENaC Dysfunction in Human Airway Epithelial Cells.
Exp Cell Res
Calreticulin positively regulates the expression and function of epithelial sodium channel.
Activity-based training reverses SCI-induced changes in kidney receptor densities and membrane proteins.
Melatonin Attenuates Sepsis-Induced Acute Lung Injury Through Improvement of Epithelial Sodium Channel-Mediated Alveolar Fluid Clearance Via Activation of SIRT1/SGK1/Nedd4-2 Signaling Pathway.