RyDEN Polyclonal antibody

• 

  WB analysis of THP-1 using 27865-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 27865-1-AP (RyDEN antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27865-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27865-1-AP (RyDEN antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

RyDEN Polyclonal Antibody for WB, ELISA

Cat No. 27865-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat and More (2)

Applications

WB, ELISA

Synonyms

C19orf66, Repressor of yield of DENV protein, RyDEN, SFL, SHFL, shiftless, UPF0515 protein C19orf66

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Tested Applications

• Positive WB detected in: THP-1 cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

27865-1-AP targets RyDEN in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26838

  Product name: Recombinant human C19orf66 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-255 aa of BC035817

  Sequence: MSQEGVELEKSVRRLREKFHGKVSSKKAGALMRKFGSDHTGVGRSIVYGVKQKDGQELSNDLDAQDPPEDMKQDRDIQAVATSLLPLTEANLRMFQRAQDDLIPAVDRQFACSSCDHVWWRRVPQRKEVSRCRKCRKRYEPVPADKMWGLAEFHCPKCRHNFRTHTHSCSAADCYNRREPHVPGTSCAHPKSRKQNHLPKVLHPSNPHISSGSTVATCLSQGGLLEDLDNLILEDLKEEEEEEEEVEDEEGGPRE

• Full Name: chromosome 19 open reading frame 66
• Observed Molecular Weight: 38 kDa
• GenBank Accession Number: BC035817
• Gene Symbol: C19orf66
• Gene ID (NCBI): 55337
• RRID: AB_2880997
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NUL5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; The short isoform of the host antiviral protein ZAP acts as an inhibitor of SARS-CoV-2 programmed ribosomal frameshifting.; Authors - Matthias M Zimmer
human,mouse	WB	Proc Natl Acad Sci U S A; Shiftless inhibits flavivirus replication in vitro and is neuroprotective in a mouse model of Zika virus pathogenesis.; Authors - Natasha W Hanners ; KD Validated
human	WB	Int Immunopharmacol; C19orf66 restricts Coxsackievirus B infection by inducing lysosomal degradation of the viral proteins 3Dpol and 2Apro and exhibits neuroprotective effects in CVB-challenged mice; Authors - Yongyao Tan