Tested Applications
| Positive WB detected in | mouse liver tissue, human placenta tissue, mouse ovary tissue, mouse testis tissue, mouse spleen tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human liver cancer tissue, mouse spleen tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
This antibody is not recommended for WB detection of brain sample.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10727-1-AP targets Ferritin light chain in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, bovine, hamster, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1149 Product name: Recombinant human FTL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC008439 Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD Predict reactive species |
| Full Name | ferritin, light polypeptide |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | BC008439 |
| Gene Symbol | Ferritin light chain |
| Gene ID (NCBI) | 2512 |
| RRID | AB_2278673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02792 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| IHC protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| IP protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| WB protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Ferritin Heavy Chain antibody (Cat# 10727-1-AP) has accumulated 294 citations across high-impact journals including Nature, Cell, Cell Metabolism, Nature Cell Biology, Nature Communications, Cancer Research, Autophagy, Redox Biology, Advanced Science, Brain, Cell Death & Disease, Oncogene, and Free Radical Biology and Medicine. Research fields span ferroptosis, iron homeostasis, ferritinophagy, neurodegeneration, oncology, hepatology, nephrology, cardiovascular disease, and inflammatory disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin | ||
Cell Metab Reactive oxygen species regulation by NCF1 governs ferroptosis susceptibility of Kupffer cells to MASH | ||
Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease |
Reviews
What customers say
All three reviews are for Western Blot applications with positive ratings (4–5 stars). Users report good lane-to-lane consistency and reliable detection of the target subunit (~17–28 kDa). One reviewer noted slightly diffuse bands but attributed this to membrane transfer of small proteins rather than antibody quality. Working dilutions ranged from 1:2000 to 1:10000. Tested species include mouse and human. Overall, reviewers express satisfaction with the antibody's performance and reproducibility.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (08-21-2026) | Demonstrates good lane-to-lane consistency across samples, successfully marking the target subunit region between 17 and 28 kDa.The bands in this specific image appear somewhat diffuse, but this is entirely a technical artifact of the membrane transfer stage (common with small ~20 kDa proteins) and not a reflection of antibody quality. satisfied with this antibody.
 |
Laura (Verified Customer) (08-26-2024) | used for WB
|
Amandine (Verified Customer) (02-07-2024) | 25ug of protein DENATURED, PVDF membrane, primary antibody 1/10000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation, 6min.
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