Tested Applications
| Positive IHC detected in | human heart tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13299-1-AP targets BNP/NPPB in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4039 Product name: Recombinant human BNP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-134 aa of BC025785 Sequence: MDPQTAPSRALLLLLFLHLAFLGGRSHPLGSPGSASDLETSGLQEQRNHLQGKLSELQVEQTSLEPLQESPRPTGVWKSREVATEGIRGHRKMVLYTLRAPRSPKMVQGSGCFGRKMDRISSSSGLGCKVLRRH Predict reactive species |
| Full Name | natriuretic peptide precursor B |
| Calculated Molecular Weight | 134 aa, 15 kDa |
| GenBank Accession Number | BC025785 |
| Gene Symbol | BNP |
| Gene ID (NCBI) | 4879 |
| RRID | AB_2877935 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16860 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
NPPB, also known as B-type natriuretic peptide (BNP), is a cardiac hormone that is secreted mainly in the ventricles in response to increased wall stress. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. BNP is a marker of systolic and diastolic dysfunction and a strong predictor of mortality in heart failure patients.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BNP/NPPB antibody 13299-1-AP | Download protocol |
| IHC protocol for BNP/NPPB antibody 13299-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BNP/NPPB polyclonal antibody (Cat# 13299-1-AP) has 24 citations published in journals including Science Advances, Advanced Science, Cell Death & Disease, Cardiovascular Diabetology, Acta Pharmacologica Sinica, Biochemical Pharmacology, International Immunopharmacology, Cell Signaling, iScience, Frontiers in Pharmacology, European Journal of Pharmacology, BMC Cardiovascular Disorders, and others. Associated research fields primarily involve cardiac hypertrophy, heart failure, myocardial remodeling, diabetic cardiomyopathy, mitochondrial dysfunction, and cardiovascular inflammatory signaling.
| Species | Application | Title |
|---|---|---|
Cardiovasc Diabetol MCT4-dependent lactate transport: a novel mechanism for cardiac energy metabolism injury and inflammation in type 2 diabetes mellitus | ||
Adv Sci (Weinh) TRIM40 Drives Pathological Cardiac Hypertrophy and Heart Failure via Ubiquitination of PKN2. | ||
Sci Adv The immunoproteasome catalytic β5i subunit regulates cardiac hypertrophy by targeting the autophagy protein ATG5 for degradation. | ||
Acta Pharmacol Sin The solute carrier transporter SLCO5A1 as a novel therapeutic target for the treatment of myocardial infarction-induced heart failure. | ||
Phytother Res Carnosol prevents cardiac remodeling and ventricular arrhythmias in pressure overload-induced heart failure mice |
