Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14361-1-AP targets Adiponectin receptor in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5744 Product name: Recombinant human ADIPOR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC051858 Sequence: MNEPTENRLGCSRTPEPDIRLRKGHQLDGTRRGDNDSHQGDLEPILEASVLSSHHKKSSEEHEYSDEAPQEDEGFMGMSPLLQAHHAMEKMEEFVCKVWEGRWRVIPHDVLPDWLKDNDFLLHGHRPPMPSFRACFKSIFRIHTETG Predict reactive species |
| Full Name | adiponectin receptor 2 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 44 kDa, 60 kDa |
| GenBank Accession Number | BC051858 |
| Gene Symbol | Adiponectin receptor |
| Gene ID (NCBI) | 79602 |
| RRID | AB_2222052 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86V24 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Adiponectin is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Two receptors for adiponectin (ADIPOR1 and ADIPOR2) have been identified. They mediate increased AMPK, MAPK, and PPARA ligand activity in response to adiponectin. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. The human ADIPOR2 gene maps to chromosome 12p13.31, and encodes a 386-amino acid protein with a molecular weight of 44 kDa. AdipoR2 may also exist as stable ~ 60 kDa dimers (PMID: 18842004). This antibody raised against 1-147aa of human AdipoR2 may cross-react with AdipoR1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Adiponectin receptor antibody 14361-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Adiponectin receptor (ADIPOR2) polyclonal antibody (14361-1-AP) has 13 citations across journals including Nature Communications, International Journal of Biological Sciences, Phytomedicine, Acta Pharmacologica Sinica, EMBO Journal, Food & Function, Molecular Neurobiology, Cancer Science, Journal of Clinical Medicine, Animal Biotechnology, and Biology Open. Research fields span acute liver failure, pancreatic cancer, diabetic cardiomyopathy, cardiac ischemia-reperfusion injury, lipid metabolism, skeletal muscle fiber type transformation, cognitive dysfunction, cancer biomarkers, Alzheimer's disease, and NAFLD.
| Species | Application | Title |
|---|---|---|
Nat Commun Selectively targeting the AdipoR2-CaM-CaMKII-NOS3 axis by SCM-198 as a rapid-acting therapy for advanced acute liver failure | ||
Int J Biol Sci Adiponectin Suppresses Human Pancreatic Cancer Growth through Attenuating the β-Catenin Signaling Pathway.
| ||
Phytomedicine Ginsenoside Rc inhibits diabetic cardiomyopathy by reducing lipotoxicity through the adiponectin and autophagy pathways. | ||
Acta Pharmacol Sin Circular RNA circDhx32 promotes cardiac inflammatory responses in mouse cardiac ischemia-reperfusion injury via binding to FOXO1 competed with AdipoR1
| ||
EMBO J Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2
| ||
Food Funct Dietary leucine and fish oil cooperatively regulate skeletal myofiber type transformation via the CaMKII signaling pathway of pigs |
