"Albumin Antibodies" Comparison
View side-by-side comparison of Albumin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
| Positive WB detected in | human blood tissue, human plasma, mouse serum, HepG2 cells, mouse liver tissue, rat liver tissue |
| Positive IP detected in | human plasma tissue |
| Positive IHC detected in | human colon tissue, human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse liver tissue |
| Positive IF/ICC detected in | L02 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16475-1-AP targets Albumin in WB, IHC, IF/ICC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9569 Product name: Recombinant human Albumin protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 256-609 aa of BC034023 Sequence: SKLVTDLTKVHTECCHGDLLECADDRADLAKYICENQDSISSKLKECCEKPLLEKSHCIAEVENDEMPADLPSLAADFVESKDVCKNYAEAKDVFLGMFLYEYARRHPDYSVVLLLRLAKTYETTLEKCCAAADPHECYAKVFDEFKPLVEEPQNLIKQNCELFEQLGEYKFQNALLVRYTKKVPQVSTPTLVEVSRNLGKVGSKCCKHPEAKRMPCAEDYLSVVLNQLCVLHEKTPVSDRVTKCCTESLVNRRPCFSALEVDETYVPKEFNAETFTFHADICTLSEKERQIKKQTALVELVKHKPKATKEQLKAVMDDFAAFVEKCCKADDKETCFAEEGKKLVAASQAALGL Predict reactive species |
| Full Name | albumin |
| Calculated Molecular Weight | 609 aa, 69 kDa |
| Observed Molecular Weight | 66 kDa |
| GenBank Accession Number | BC034023 |
| Gene Symbol | Albumin |
| Gene ID (NCBI) | 213 |
| RRID | AB_2242567 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02768 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Albumin is the most abundant protein in blood plasma. Alterations of level of serum albumin are linked to variety of diseases. Albumin is expressed exclusively by well-differentiated hepatocytes, thus anti-albumin has been used to mark hepatocytes. In additon, glycated serum albumin is also a potential diabetes biomarker. The N-terminal 24aa of albumin will be cleaved to generate the mature form of serum albumin with MW 66 kDa. (21388516, 23832071)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Albumin antibody 16475-1-AP | Download protocol |
| IHC protocol for Albumin antibody 16475-1-AP | Download protocol |
| IP protocol for Albumin antibody 16475-1-AP | Download protocol |
| WB protocol for Albumin antibody 16475-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Albumin (ALB) Antibody (Cat# 16475-1-AP) has accumulated 266 total citations. Citations appear in high-impact journals including Nature, Science, Cell, Signal Transduction and Targeted Therapy, Cell Metabolism, Nature Communications, Immunity, Hepatology, Neuron, and over 80 additional journals. Research fields span liver diseases (NAFLD/NASH, fibrosis, acute liver failure), lipid metabolism, extracellular vesicles/exosomes, blood-brain barrier biology, neuroinflammation, oncology (hepatocellular, colorectal, pancreatic carcinoma), diabetes, cardiovascular disease, immunology, stem cell/organoid research, and ferroptosis.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Albumosomes formed by cytoplasmic pre-folding albumin maintain mitochondrial homeostasis and inhibit nonalcoholic fatty liver disease | ||
Signal Transduct Target Ther MDIG-mediated H3K9me3 demethylation upregulates Myc by activating OTX2 and facilitates liver regeneration | ||
Signal Transduct Target Ther The AKAP12-PKA axis regulates lipid homeostasis during alcohol-associated liver disease | ||
Science Enterically derived high-density lipoprotein restrains liver injury through the portal vein. | ||
Cell snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification |
Reviews
What customers say
Users report strong performance across multiple applications. For immunofluorescence, it works well at 1:100–1:300 on mouse tissues, though one user noted secondary antibody choice is critical (Alexa Fluor 555 worked while 488 did not). Western blot showed good results at 1:25,000 dilution. Immunohistochemistry on mouse liver tissue stained well at 1:200. Overall, the antibody is well-received with an average rating of 4.5/5, with the main caveat being sensitivity to secondary selection in IF experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
MALLIKARJUNA (Verified Customer) (10-14-2025) | NICE ANTIBODY FOR IF
|
Martin (Verified Customer) (10-09-2025) | Tested on mouse muscle cross-sections (acetone-fixed). The antibody only gave a consisted signal with my Alexa Fluor 555 secondary; no staining observed with 488. Works, but secondary choice seems critical.
|
Kamal (Verified Customer) (01-26-2024) | For western blot analysis, antibody worked well at dilution of 1:25000 in 1 X TBST. For immunofluorescence, antibody worked well at dilution of 1:200 in 1 X PBS.
|
Kamal (Verified Customer) (01-26-2024) | Liver tissues were well stained in provided dilution with 1X PBS.
|
