EIF2AK2, PKR Polyclonal antibody

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• EIF2AK2, PKR Polyclonal antibody (18244-1-AP)

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• KD/KO Validated

EIF2AK2, PKR Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18244-1-AP Copied!

Publications (70)

Reviews (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

EIF2AK2, PKR, Interferon-induced, double-stranded RNA-activated protein kinase, Eukaryotic translation initiation factor 2-alpha kinase 2, eIF-2A protein kinase 2

• 

  WB analysis using 18244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 18244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 18244-1-AP

  WB result of EIF2AK2, PKR antibody (18244-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EIF2AK2, PKR transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of rat heart using 18244-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 18244-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse thymus using 18244-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 18244-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 18244-1-AP

  IP result of anti-EIF2AK2, PKR (IP:18244-1-AP, 4ug; Detection:18244-1-AP 1:500) with HeLa cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 18244-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 18244-1-AP (EIF2AK2, PKR antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 18244-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EIF2AK2, PKR antibody (18244-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A549 cells, Jurkat cells, mouse thymus tissue, HepG2 cells, HeLa cells, rat heart tissue, K-562 cells, MCF-7 cells, SH-SY5Y cells, THP-1 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

18244-1-AP targets EIF2AK2, PKR in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, rabbit, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13010

  Product name: Recombinant human EIF2AK2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-373 aa of BC093676

  Sequence: MAGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGT

• Full Name: eukaryotic translation initiation factor 2-alpha kinase 2
• Calculated Molecular Weight: 551 aa, 62 kDa
• Observed Molecular Weight: 65-68 kDa
• GenBank Accession Number: BC093676
• Gene Symbol: EIF2AK2
• Gene ID (NCBI): 5610
• RRID: AB_2246451
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P19525
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Phospho-eIF2α (Ser51) Antibody (Cat# 18244-1-AP) has 70 citations across journals including Nature, Cell, Cancer Cell, Nat Commun, Mol Cell, EMBO Rep, FASEB J, J Virol, and Cell Rep. Associated research fields encompass oncology (metastasis, tumorigenesis), virology (HSV, cardiovirus, SARS-CoV-2, African swine fever), neuroscience (Alzheimer's, spinal cord injury), innate immunity and interferon signaling, stress granule biology, the integrated stress response, liver disease, and metabolism.

Automatically generated from the 70 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
		Nature; Ageing promotes metastasis via activation of the integrated stress response; Authors - Angana A H Patel
human	WB	Cell; Dynamic mapping of proteome trafficking within and between living cells by TransitID; Authors - Wei Qin
human	WB	J Hepatol; eIF3f promotes tumour malignancy by remodelling fatty acid biosynthesis in hepatocellular carcinoma; Authors - Suiqing Zhou ; KD Validated
		Cell Mol Immunol; ZBP1 mediates interferon-induced necroptosis.; Authors - Daowei Yang
mouse	WB	Nat Neurosci; Maternal immune activation in mice disrupts proteostasis in the fetal brain.; Authors - Brian T Kalish

Reviews

5.0 out of 5

3 verified customer reviews

What customers say

All three reviews are 5-star and highly positive. Users report the antibody performs well for Western Blot at a 1:1000 dilution, with strong and specific signals. One user also confirmed reliable results in Immunofluorescence staining. Tested cell lines/tissues include HEK-293T, HEK-293, and mouse heart. Overall, reviewers describe the product as working very well and highly recommend it.

Automatically generated from the 3 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Vikas (Verified Customer) (12-23-2024)

Used for WB, works very good.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK-293T

RASHMI (Verified Customer) (11-27-2024)

Used for WB, Highly recommended

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK-293

Wei (Verified Customer) (03-12-2020)

Strong and specific signal for both western blot and staining experiments.

• Applications: Western Blot, Immunofluorescence,
• Cell Tissue Type: Mouse Heart