2019-nCOV nucleocapsid phosphoprotein Recombinant antibody

2019-nCOV nucleocapsid phosphoprotein Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

8C20

Cat no : 80027-1-RR

Synonyms

2019-nCOV nucleocapsid phosphoprotein, N protein, nucleocapsid phosphoprotein



Tested Applications

Positive WB detected in真核N蛋白, Ag30676
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

80027-1-RR targets 2019-nCOV nucleocapsid phosphoprotein in WB, ELISA applications and shows reactivity with virus samples.

Tested Reactivity virus
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen 2019-nCOV nucleocapsid phosphoprotein fusion protein Ag30676
Full Name COVID-19 N Protein
GenBank accession numberNC_045512
Gene symbol COVID-19 N Protein
Gene ID (NCBI) 43740575
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.

Protocols

Product Specific Protocols
WB protocol for 2019-nCOV nucleocapsid phosphoprotein antibody 80027-1-RRDownload protocol
Standard Protocols
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