SARS-CoV-2 Nucleocapsid Phosphoprotein Recombinant antibody
SARS-CoV-2 Nucleocapsid Phosphoprotein Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
virus
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
8C20
Cat no : 80027-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Eukaryotic nucleocapsid phosphoprotein, Ag30676 |
Positive ELISA detected in | Recombinant protein |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Enzyme-linked Immunosorbent Assay (ELISA) | ELISA : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
80027-1-RR targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with virus samples.
Tested Reactivity | virus |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676 |
Full Name | COVID-19 N Protein |
GenBank Accession Number | NC_045512 |
Gene Symbol | COVID-19 N Protein |
Gene ID (NCBI) | 43740575 |
RRID | AB_2882945 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.
Protocols
Product Specific Protocols | |
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WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 80027-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |