SARS-CoV-2 Nucleocapsid Phosphoprotein Recombinant antibody

SARS-CoV-2 Nucleocapsid Phosphoprotein Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

8C20

Cat no : 80027-1-RR

Synonyms

2019-nCOV nucleocapsid phosphoprotein, N protein, nucleocapsid phosphoprotein



Tested Applications

Positive WB detected inEukaryotic nucleocapsid phosphoprotein, Ag30676
Positive ELISA detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80027-1-RR targets SARS-CoV-2 Nucleocapsid Phosphoprotein in WB, ELISA applications and shows reactivity with virus samples.

Tested Reactivity virus
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SARS-CoV-2 Nucleocapsid Phosphoprotein fusion protein Ag30676
Full Name COVID-19 N Protein
GenBank Accession NumberNC_045512
Gene Symbol COVID-19 N Protein
Gene ID (NCBI) 43740575
RRIDAB_2882945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The nucleocapsid (N) protein has multiple functions including formation of nucleocapsids, signal transduction virus budding, RNA replication, and mRNA transcription. N protein is an important antigen for coronavirus, and it is normally highly conserved, with a molecular weight of about 50 kDa. it can be used as a marker in diagnostic assays due to its high immunogenicity (PMID: 32416961, PMID: 32235387). A sandwich ELISA for COVID-19 N Protein can be assembled by using 80027-1-RR as capture antibody and conjugated 80026-1-RR for detection.

Protocols

Product Specific Protocols
WB protocol for SARS-CoV-2 Nucleocapsid Phosphoprotein antibody 80027-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols