Tested Applications
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20645-1-AP targets Fibrinogen Alpha Chain in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9567 Product name: Recombinant human FGA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 20-218 aa of BC020764 Sequence: ADSGEGDFLAEGGGVRGPRVVERHQSACKDSDWPFCSGEDWNYKCPSGCRMKGLIDEVNQDFTNRINKLKNSLFEYQKNNKDSHSLTTNIMEILRGDFSSANNRDNTYNRVSEDLRSRIEVLKRKVIEKVTANNLLVARVTTEETPHLKARAIKWQMRPEVKPIMKEHIAPREAMLNLALSEVSTLLLWGSLPCPPRLS Predict reactive species |
| Full Name | fibrinogen alpha chain |
| Calculated Molecular Weight | 866 aa, 95 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC020764 |
| Gene Symbol | Fibrinogen Alpha Chain |
| Gene ID (NCBI) | 2243 |
| RRID | AB_2878715 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02671 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fibrinogen is a soluble plasma glycoprotein synthesized in the liver. It is composed of two sets of three structurally different subunits: alpha (FGA), beta (FGB), gamma (FGG). Fibrinogen is converted by thrombin into fibrin during blood coagulation. Fibrinogen and fibrin play overlapping roles in blood clotting, fibrinolysis, cellular and matrix interactions, the inflammatory response, wound healing, and neoplasia (PMID: 16102057). FGA is the alpha chain of fibrinogen. During conversion of fibrinogen to fibrin, fibrinopeptide A is cleaved from FGA by thrombin.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Fibrinogen Alpha Chain antibody 20645-1-AP | Download protocol |
| WB protocol for Fibrinogen Alpha Chain antibody 20645-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Fibrinogen Alpha Chain Polyclonal antibody (Cat# 20645-1-AP) has 23 citations appearing in journals including Nature, Nature Communications, ACS Nano, Cardiovascular Diabetology, Free Radical Biology and Medicine, Journal of Thrombosis and Haemostasis, Frontiers in Immunology, and Genes Disease. Associated research fields span hepatocellular carcinoma, sepsis-induced lung injury, coagulation and thrombosis, wound healing, angiogenesis, ischemic stroke, renal cell carcinoma, and cardiometabolic disease.
| Species | Application | Title |
|---|---|---|
Exploration (Beijing) Targeting eEF1A1 With Baicalein to Block eEF1A1/Prdx4 Interaction for Treating Sepsis-Mediated Lung Injury | ||
Nat Commun Identifying fibroblast-derived sFRP2 as a therapeutic target and engineering siRNA therapy for uterine scarring | ||
Nat Commun Controllable gelation of artificial extracellular matrix for altering mass transport and improving cancer therapies. | ||
ACS Nano Nanoscaled Titanium Oxide Layer Provokes Quick Osseointegration on 3D-Printed Dental Implants: A Domino Effect Induced by Hydrophilic Surface |
Reviews
What customers say
Both reviewers report positive results. One user achieved strong immunofluorescence staining of fibrinogen in mouse stroke brain cryosections at a 1/200 dilution with overnight incubation at 4°C, rating the product 5 stars. The other user successfully labeled fibrinogen in choroid plexus tissue by IHC and highlighted excellent stability, noting the antibody remained functional after one year stored at −20°C without aliquoting, giving it 4 stars. Overall, users are satisfied with performance and storage stability.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marion (Verified Customer) (07-15-2025) | Immunohistofluorescence (PFA-fixed cryosections) image of Fibrinogen Alpha Chain stained mouse stroke brain. Blocking step : 10 % Donkey Serum + 1% BSA + 0.02% Triton as blocking agent for 2 hours at RT Primary antiboby incubation time : 1 night / 4°C Secondary antibody : AlexaFluor 568 (ab175470 ; Abcam) ; 1/200
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Lillian (Verified Customer) (05-27-2025) | Labeled fibrinogen in our tissue samples. Kept at -20C for the past year and worked even when not aliquoted.
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