Tested Applications
| Positive WB detected in | HeLa cells, rat liver tissue, Raji cells, Jurkat cells, HepG2 cells, L02 cells, mouse liver tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human liver cancer tissue, human hepatocirrhosis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21260-1-AP targets Catalase in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, pig, chicken, bovine, goat, bat, fungus, meretrix meretrix |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15383 Product name: Recombinant human Catalase protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 228-527 aa of BC112219 Sequence: EAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL Predict reactive species |
| Full Name | catalase |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC112219 |
| Gene Symbol | Catalase |
| Gene ID (NCBI) | 847 |
| RRID | AB_10733099 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04040 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Catalase belongs to the catalase family. CAT occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. CAT promotes the growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells, and normal and transformed fibroblast cells. CAT catalyzes the reaction: 2 H2O2 = O2 + 2 H2O. Defects in CAT are the cause of acatalasia (ACATLAS) which is also known as acatalasemia.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Catalase antibody 21260-1-AP | Download protocol |
| IP protocol for Catalase antibody 21260-1-AP | Download protocol |
| WB protocol for Catalase antibody 21260-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Catalase antibody (Cat# 21260-1-AP) has accumulated 259 citations across high-impact journals including Nature Communications, Cell Metabolism, Advanced Materials, Nature Aging, Biomaterials, Free Radical Biology and Medicine, Oxidative Medicine and Cellular Longevity, Antioxidants & Redox Signaling, Cell Death & Disease, and Redox Biology. Research fields span oxidative stress, ferroptosis, mitochondrial homeostasis, diabetes, cancer, neuroprotection, cardiovascular disease, aging, renal injury, bone regeneration, liver fibrosis, wound healing, and reproductive biology, with applications predominantly in Western blotting.
| Species | Application | Title |
|---|---|---|
Nanomicro Lett A Rational Design of Metal-Organic Framework Nanozyme with High-Performance Copper Active Centers for Alleviating Chemical Corneal Burns | ||
Adv Mater Functionalized Nanozyme Microcapsules Targeting Deafness Prevention via Mitochondrial Homeostasis Remodeling | ||
Nat Aging Bifidobacterium adolescentis regulates catalase activity and host metabolism and improves healthspan and lifespan in multiple species | ||
Nat Commun Intelligence-responsive wettability switch coating on magnesium implants for treating osteoporotic fracture. |
Reviews
What customers say
All four reviews (4–5 stars) report successful use of this antibody for Western Blot at 1:2000 dilution. Users confirmed clear detection of catalase in human fibroblasts and HepG2 cells, with one noting it worked even at very low protein amounts (0.01 µg). The antibody proved robust to accidental 24-hour room-temperature storage. One reviewer observed mild background with BSA blocking and suggested a higher concentration for cell lysates to improve signal. Overall, reviewers found it reliable and well-performing for catalase detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Silvia (Verified Customer) (08-11-2022) | It worked well for western blotting.
|
Maximilian (Verified Customer) (06-03-2022) | The antibody works fine and detected human catalase at 0.01 and 0.05 µg total protein amount in my blot. Accidental storage time of 24h at RT did not affect performance.
|
Maximilian (Verified Customer) (05-27-2022) | Antibody performed well in detection of catalase standard protein (catalase from human erythrocytes) but signal for cell lysates could be better (will increase antibody concentration). Maybe this is due to the storage for 24h at room temperature, which was the result of wrong storage by a colleague, as the package was erroneously delivered to her instead of me.
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Tom (Verified Customer) (08-25-2021) | Membrane blocked in BSA solution prior to incubation with antibody (1:2000). Correct band is clearly visible, but with some background.
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