Alpha 1B-Glycoprotein Polyclonal antibody

Alpha 1B-Glycoprotein Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14181-1-AP

Synonyms

A1B, A1BG, ABG, alpha 1 B glycoprotein, Alpha 1B glycoprotein, GAB, HYST2477



Tested Applications

Positive WB detected inhuman plasma tissue, BxPC-3 cells, MCF-7 cells, mouse kidney tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

14181-1-AP targets Alpha 1B-Glycoprotein in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alpha 1B-Glycoprotein fusion protein Ag5414
Full Name alpha-1-B glycoprotein
Calculated molecular weight 54 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC035719
Gene symbol A1BG
Gene ID (NCBI) 1
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Alpha-1B-glycoprotein (A1BG) is a plasma protein and is a member of the immunoglobulin superfamily. A1BG contains 474 amino acids and has 5 intrachain disulfide bonds. It has been reported that cysteine-rich secretory protein 3 (CRISP-3) is a specific and high-affinity ligand of A1BG. A1BG-CRISP-3 complex displays a similar function in protecting the circulation from a potentially harmful effect of free CRISP3 (PMID: 15461460).

Protocols

Product Specific Protocols
WB protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
IHC protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
IF protocol for Alpha 1B-Glycoprotein antibody 14181-1-APDownload protocol
Standard Protocols
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