A4GALT Polyclonal antibody, PBS Only

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• A4GALT Polyclonal antibody, PBS Only (12392-1-PBS)

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  WB analysis of RAW 264.7 using 12392-1-AP (same clone as 12392-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 12392-1-AP (same clone as 12392-1-PBS)

  Raji cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 12392-1-AP (same clone as 12392-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 12392-1-AP (A4GALT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human cervical cancer using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 12392-1-AP (same clone as 12392-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 12392-1-AP (A4GALT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12392-1-AP (same clone as 12392-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using A4GALT antibody (12392-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12392-1-PBS in a different storage buffer formulation.

A4GALT Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 12392-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

12392-1-PBS targets A4GALT in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3069

  Product name: Recombinant human A4GALT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 41-353 aa of BC017068

  Sequence: HVVGEPKEKGQLYNLPAEIPCPTLTPPTPPSHGPTPGNIFFLETSDRTNPNFLFMCSVESAARTHPESHVLVLMKGLPGGNASLPRHLGISLLSCFPNVQMLPLDLRELFRDTPLADWYAAVQGRWEPYLLPVLSDASRIALMWKFGGIYLDTDFIVLKNLRNLTNVLGTQSRYVLNGAFLAFERRHEFMALCMRDFVDHYNGWIWGHQGPQLLTRVFKKWCSIRSLAESRACRGVTTLPPEAFYPIPWQDWKKYFEDINPEELPRLLSATYAVHVWNKKSQGTRFEATSRALLAQLHARYCPTTHEAMKMYL

• Full Name: alpha 1,4-galactosyltransferase
• Calculated Molecular Weight: 353 aa, 41 kDa
• Observed Molecular Weight: 50-52 kDa
• GenBank Accession Number: BC017068
• Gene Symbol: A4GALT
• Gene ID (NCBI): 53947
• RRID: AB_2877853
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NPC4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.