Tested Applications
| Positive IHC detected in | mouse ovary tissue, mouse testis tissue, rat ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17990-1-AP targets AANAT in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12422 Product name: Recombinant human AANAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-207 aa of BC092430 Sequence: MSTQSTHPLKPEAPRLPPGIPESPSCQRRHTLPASEFRCLTPEDAVSAFEIEREAFISVLGVCPLYLDEIRHFLTLCPELSLGWFEEGCLVAFIIGSLWDKERLMQESLTLHRSGGHIAHLHVLAVHRAFRQQGRGPILLWRYLHHLGSQPAVRRAALMCEDALVPFYERFSFHAVGPCAITVGSLTFMELHCSLRGHPFLRRNSGC Predict reactive species |
| Full Name | arylalkylamine N-acetyltransferase |
| Calculated Molecular Weight | 207 aa, 23 kDa |
| GenBank Accession Number | BC092430 |
| Gene Symbol | AANAT |
| Gene ID (NCBI) | 15 |
| RRID | AB_2878477 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16613 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Serotonin N-acetyltransferase (arylalkylamine N-acetyltransferase; AANAT), an indoleamine originally found in the pineal gland, is considered as an antioxidant or a free radical scavenger, and promotes the synthesis of glutathione, which is an antioxidant. This enzyme catalyzes the metabolism of the neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) into Nacetylserotonin (NAS) an intermediate in the synthesis of melatonin and there is an evidence for the neuronal presence of NAS in the mammalian brain.(PMID:11985883; 20488463)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for AANAT antibody 17990-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is MT2 (Melatonin Receptor MT2), catalog number 17990-1-AP, with 11 total citations. Citations appear in journals including Gut Microbes, J Pineal Res, Environ Pollut, J Agric Food Chem, Cells, Int Immunopharmacol, Invest Ophthalmol Vis Sci, Exp Neurol, Front Physiol, World J Psychiatry, and PLoS One. Research fields span melatonin/circadian biology, reproductive endocrinology, environmental toxicology, gut microbiota, oxidative stress, immunology, ophthalmology, neurology, photobiology, psychiatry, and mitochondrial/autophagy biology.
| Species | Application | Title |
|---|---|---|
Gut Microbes Gut microbiota regulates host melatonin production through epithelial cell MyD88 | ||
Environ Pollut Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction. | ||
J Agric Food Chem Maternal Long-Term Intake of Inulin Improves Fetal Development through Gut Microbiota and Related Metabolites in a Rat Model. | ||
Cells Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway. | ||
Int Immunopharmacol Melatonin relieves Th17/CD4-CD8- T cells inflammatory responses via nuclear-receptor dependent manner in peripheral blood of primary Sjögren's syndrome. |
