AANAT Polyclonal antibody

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  IHC staining of mouse ovary using 17990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17990-1-AP (AANAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 17990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17990-1-AP (AANAT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 17990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 17990-1-AP (AANAT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 17990-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17990-1-AP (AANAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AANAT Polyclonal Antibody for IHC, ELISA

Cat No. 17990-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

IHC, IF, ELISA

Synonyms

AA NAT, AANAT, Serotonin acetylase, Serotonin N acetyltransferase, SNAT

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Tested Applications

• Positive IHC detected in: mouse ovary tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

17990-1-AP targets AANAT in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken, hamster
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12422

  Product name: Recombinant human AANAT protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-207 aa of BC092430

  Sequence: MSTQSTHPLKPEAPRLPPGIPESPSCQRRHTLPASEFRCLTPEDAVSAFEIEREAFISVLGVCPLYLDEIRHFLTLCPELSLGWFEEGCLVAFIIGSLWDKERLMQESLTLHRSGGHIAHLHVLAVHRAFRQQGRGPILLWRYLHHLGSQPAVRRAALMCEDALVPFYERFSFHAVGPCAITVGSLTFMELHCSLRGHPFLRRNSGC

• Full Name: arylalkylamine N-acetyltransferase
• Calculated Molecular Weight: 207 aa, 23 kDa
• GenBank Accession Number: BC092430
• Gene Symbol: AANAT
• Gene ID (NCBI): 15
• RRID: AB_2878477
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16613
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Environ Pollut; Exogenous melatonin protects preimplantation embryo development from decabromodiphenyl ethane-induced circadian rhythm disorder and endogenous melatonin reduction.; Authors - Feifei Shi
rat		Exp Neurol; MiR-375-3p mediates reduced pineal function in hypoxia-ischemia brain damage.; Authors - Lixiao Xu
mouse		Cells; Activation of MT1/MT2 to Protect Testes and Leydig Cells against Cisplatin-Induced Oxidative Stress through the SIRT1/Nrf2 Signaling Pathway.; Authors - Junqiang Zhang
rat		J Agric Food Chem; Maternal Long-Term Intake of Inulin Improves Fetal Development through Gut Microbiota and Related Metabolites in a Rat Model.; Authors - Xie Peng
human	IHC	Int Immunopharmacol; Melatonin relieves Th17/CD4-CD8- T cells inflammatory responses via nuclear-receptor dependent manner in peripheral blood of primary Sjögren's syndrome.; Authors - Yi Liu
		Front Physiol; Photoperiod Affects Harderian Gland Morphology and Secretion in Female Cricetulus barabensis: Autophagy, Apoptosis, and Mitochondria.; Authors - Zhe Wang