Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22696-1-AP targets AARS2 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18539 Product name: Recombinant human AARS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 635-985 aa of BC131728 Sequence: THLLNWALRQTLGPGTEQQGSHLNPEQLRLDVTTQTPLTPEQLRAVENTVQEAVGQDEAVYMEEVPLALTAQVPGLRSLDEVYPDPVRVVSVGVPVAHALDPASQAALQTSVELCCGTHLLRTGAVGDLVIIGDRQLSKGTTRLLAVTGEQAQQARELGQSLAQEVKAATERLSLGSRDVAEALRLSKDIGRLIEAVETAVMPQWQRRELLATVKMLQRRANTAIRKLQMGQAAKKTQELLERHSKGPLIVDTVSAESLSVLVKVVRQLCEQAPSTSVLLLSPQPMGKVLCACQVAQGAMPTFTAEAWALAVCSHMGGKAWGSRVVAQGTGSTTDLEAALSIAQTYALSQL Predict reactive species |
| Full Name | alanyl-tRNA synthetase 2, mitochondrial (putative) |
| Calculated Molecular Weight | 985 aa, 107 kDa |
| Observed Molecular Weight | 105-115 kDa |
| GenBank Accession Number | BC131728 |
| Gene Symbol | AARS2 |
| Gene ID (NCBI) | 57505 |
| RRID | AB_11182926 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5JTZ9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
AARS2(Alanine--tRNA ligase, mitochondrial) is also named as KIAA1270, AARSL, bA444E17.1, AlaRS, AARSL, COXPD8, MTALARS, MT-ALARS and belongs to the class-II aminoacyl-tRNA synthetase family. It catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). It also edits incorrectly charged tRNA(Ala) via its editing domain. The full length 107 kDa protein has a transit peptide with 23 amino acids.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for AARS2 antibody 22696-1-AP | Download protocol |
| IHC protocol for AARS2 antibody 22696-1-AP | Download protocol |
| IP protocol for AARS2 antibody 22696-1-AP | Download protocol |
| WB protocol for AARS2 antibody 22696-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The AARS2 Polyclonal antibody (Cat# 22696-1-AP) has 13 citations published in journals including Cell Research, Nature Communications, Cell Stem Cell, Molecular Cancer, Autophagy, EMBO Molecular Medicine, Nucleic Acids Research, Journal of Hematology & Oncology, Frontiers in Immunology, Cancer Medicine, International Journal of Biological Macromolecules, and World Journal of Gastrointestinal Surgery. Research fields span protein lactylation, mitochondrial biology, oncology (renal cell carcinoma, bladder cancer, lymphoma), cardiomyopathy, autophagy, ferroptosis, and immunology.
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Integrated proteogenomic characterization of urothelial carcinoma of the bladder. | ||
Mol Cancer Targeting MYC in T-Cell lymphoma via epigenetic modulation with a small-molecule inhibitor. | ||
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation
| ||
Cell Stem Cell A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration. | ||
Autophagy AARS2-mediated lactylation of ULK1 promotes autophagy-dependent progression of clear cell renal cell carcinoma. | ||
Nat Commun NPM3 functions as a lactyltransferase to promote necroptosis in male diabetic cardiomyopathy mice models via FASN transcription modulation. |
