AARSD1 Polyclonal antibody, PBS Only

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• AARSD1 Polyclonal antibody, PBS Only (14900-1-PBS)

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  WB analysis using 14900-1-AP (same clone as 14900-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14900-1-AP (AARSD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14900-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14900-1-AP (same clone as 14900-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14900-1-AP (AARSD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14900-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 14900-1-AP (same clone as 14900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 14900-1-AP (same clone as 14900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14900-1-AP (same clone as 14900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14900-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14900-1-AP (same clone as 14900-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14900-1-AP (AARSD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14900-1-PBS in a different storage buffer formulation.

AARSD1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 14900-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

14900-1-PBS targets AARSD1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6692

  Product name: Recombinant human AARSD1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 176-525 aa of BC004172

  Sequence: TINDISVLRVTRRGEQADHFTQTPLDPGSQVLVRVDWERRFDHMQQHSGQHLITAVADHLFKLKTTSWELGRFRSAIELDTPSMTAEQVAAIEQSVNEKIRDRLPVNVRELSLDDPEVEQVSGRGLPDDHAGPIRVVNIEGVDSNMCCGTHVSNLSDLQVIKILGTEKGKKNRTNLIFLSGNRVLKWMERSHGTEKALTALLKCGAEDHVEAVKKLQNSTKILQKNNLNLLRDLAVHIAHSLRNSPDWGGVVILHRKEGDSEFMNIIANEIGSEETLLFLTVGDEKGGGLFLLAGPPASVETLGPRVAEVLEGKGAGKKGRFQGKATKMSRRMEAQALLQDYISTQSAKE

• Full Name: alanyl-tRNA synthetase domain containing 1
• Calculated Molecular Weight: 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC004172
• Gene Symbol: AARSD1
• Gene ID (NCBI): 80755
• RRID: AB_2257630
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BTE6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AARSD1(Alanyl-tRNA synthetase domain-containing protein 1) is also named as alanyl-tRNA editing protein Aarsd1 and belongs to the class-II aminoacyl-tRNA synthetase family. It plays a role in prevention of synthesis of proteins containing errors (mistranslation)(PMID:18172502). It has 3 isoforms produced by alternative splicing with the molecular weight of 45 kDa, 59 kDa and 66 kDa.