Tested Applications
| Positive WB detected in | HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | Customer Sample customer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11349-1-AP targets ABAT in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1877 Product name: Recombinant human ABAT protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 201-500 aa of BC015628 Sequence: QAPGCPDYSILSFMGAFHGRTMGCLATTHSKAIHKIDIPSFDWPIAPFPRLKYPLEEFVKENQQEEARCLEEVEDLIVKYRKKKKTVAGIIVEPIQSEGGDNHASDDFFRKLRDIARKHGCAFLVDEVQTGGGCTGKFWAHEHWGLDDPADVMTFSKKMMTGGFFHKEEFRPNAPYRIFNTWLGDPSKNLLLAEVINIIKREDLLNNAAHAGKALLTGLLDLQARYPQFISRVRGRGTFCSFDTPDDSIRNKLILIARNKGVVLGGCGDKSIRFRPTLVFRDHHAHLFLNIFSDILADFK Predict reactive species |
| Full Name | 4-aminobutyrate aminotransferase |
| Calculated Molecular Weight | 500 aa, 56 kDa |
| Observed Molecular Weight | 50 kDa |
| GenBank Accession Number | BC015628 |
| Gene Symbol | ABAT |
| Gene ID (NCBI) | 18 |
| RRID | AB_2220001 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P80404 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ABAT antibody 11349-1-AP | Download protocol |
| IP protocol for ABAT antibody 11349-1-AP | Download protocol |
| WB protocol for ABAT antibody 11349-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
ABAT Polyclonal Antibody (Cat# 11349-1-AP) has 10 citations published in journals including Nature Cell Biology, Journal of Experimental & Clinical Cancer Research, MedComm, Pharmaceuticals, Cell Oncology, Molecular Neurobiology, Journal of Sleep Research, Frontiers in Cellular Neuroscience, Carcinogenesis, and Cancer Investigation. Research fields span cancer biology (tumour growth, metastasis, colorectal and renal cancers), neuroscience (GABA signaling, Parkinson's disease, astrocytic function), neuroprotection, and insomnia mechanisms.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression. | ||
J Exp Clin Cancer Res GABA regulates metabolic reprogramming to mediate the development of brain metastasis in non-small cell lung cancer | ||
MedComm (2020) Neurotransmitter accumulation and Parkinson's disease-like phenotype caused by anion channelrhodopsin opto-controlled astrocytic mitochondrial depolarization in substantia nigra pars compacta | ||
Pharmaceuticals (Basel) Excavating Precursors from the Traditional Chinese Pair Herbs Polygala tenuifolia and Gastrodia elata: Synthesis, Anticonvulsant Activity Evaluation of 3,4,5-Trimethoxycinnamic Acid (TMCA) Peptide Analogs. | ||
Cell Oncol (Dordr) TRPV1 inhibition suppresses non-small cell lung cancer progression by inhibiting tumour growth and enhancing the immune response | ||
Mol Neurobiol Mechanisms of Senegenin in Regulating Oxidative Stress and Mitochondria Damage for Neuroprotection in Insomnia: Evidence from In Vivo and In Vitro Models |
Reviews
What customers say
One reviewer (Donna P.) rated this product 4/5 stars in November 2018. She used it at a 1:1000 dilution on HBMEC cells for Western Blot and reported that it "works good with minimal background." Overall, the single review is positive, highlighting clean results with low background signal in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Donna (Verified Customer) (11-26-2018) | Works good with minimal background.
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