ABAT Polyclonal antibody

ABAT Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11349-1-AP

Synonyms

ABAT, GABA aminotransferase, GABA AT, GABA T, GABA transaminase, GABAT, L AIBAT



Tested Applications

Positive WB detected inHepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

11349-1-AP targets ABAT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABAT fusion protein Ag1877
Full Name 4-aminobutyrate aminotransferase
Calculated molecular weight 500 aa, 56 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015628
Gene symbol ABAT
Gene ID (NCBI) 18
RRIDAB_2220001
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABAT antibody 11349-1-APDownload protocol
IHC protocol for ABAT antibody 11349-1-APDownload protocol
IP protocol for ABAT antibody 11349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

Nat Cell Biol

Cancer-cell-derived GABA promotes β-catenin-mediated tumour growth and immunosuppression.

Authors - De Huang
ratIF

Front Cell Neurosci

Neuronal gamma-aminobutyric acid (GABA) type A receptors undergo cognate ligand chaperoning in the endoplasmic reticulum by endogenous GABA.

Authors - Ping Wang
humanIHC

Cancer Invest

Integrative analysis developing and validating potential candidate biomarkers for cancer stemness features of pan-renal cell carcinoma

Authors - Fei Lin
mouseWB

Carcinogenesis

EphB6 deficiency in intestinal neurons promotes tumor growth in colorectal cancer by neurotransmitter GABA signaling

Authors - Hao Yu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Donna (Verified Customer) (11-26-2018)

Works good with minimal background.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HBMEC