ABCA1 Recombinant monoclonal antibody, PBS Only (Detector)

• Home
• Products
• Primary Antibodies
• Recombinant Antibodies
• ABCA1 Recombinant monoclonal antibody, PBS Only (Detector) (85434-3-PBS)

ABCA1 Uni-rAb^® Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Cat No. 85434-3-PBS

Clone No.261086C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Sandwich ELISA, Indirect ELISA

Synonyms

ABC1, ABC-1, ATP-binding cassette 1, ATP-binding cassette sub-family A member 1, ATP-binding cassette transporter 1

• 

  WB analysis using 85434-3-RR (same clone as 85434-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85434-3-RR (ABCA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85434-3-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP01937-2

  The mean ABCA1 concentration was determined to be 26.47 ng/mL in THP-1 cell extract based on a 1.20 mg/mL extract load, 15.96 ng/mL in HaCaT cell extract based on a 1.20 mg/mL extract load, 15.79 ng/mL in HEK-293 cell extract based on a 1.20 mg/mL extract load and 7.79 ng/mL in mouse brain extract based on a 1.20 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP01937-2

  The mean ABCA1 concentration was determined to be 31.41 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load, 19.14 ng/mL in Calu-3 cell extract based on a 1.20 mg/mL extract load and 19.22 ng/mL in SH-SY5Y cell extract based on a 1.20 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP01937-2

  Sandwich ELISA standard curve of MP01937-2, Human ABCA1 Recombinant Matched Antibody Pair - PBS only. 85434-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag37398. 85434-3-PBS was HRP conjugated as the detection antibody. Range: 0.625-40 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Product Information

85434-3-PBS targets ABCA1 as part of a matched antibody pair:

MP01937-2: 85434-4-PBS capture and 85434-3-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag37398

  Product name: Recombinant human ABCA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 330-550 aa of BC146856

  Sequence: YKALFGGNGTEEDAETFYDNSTTPYCNDLMKNLESSPLSRIIWKALKPLLVGKILYTPDTPATRQVMAEVNKTFQELAVFHDLEGMWEELSPKIWTFMENSQEMDLVRMLLDSRDNDHFWEQQLDGLDWTAQDIVAFLAKHPEDVQSSNGSVYTWREAFNETNQAIRTISRFMECVNLNKLEPIATEVWLINKSMELLDERKFWAGIVFTGITPGSIELPH

• Full Name: ATP-binding cassette, sub-family A (ABC1), member 1
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: BC146856
• Gene Symbol: ABCA1
• Gene ID (NCBI): 19
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O95477
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABCA, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABCA subfamily. Members of the ABCA subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesterol efflux pump in the cellular lipid removal pathway.