ABCB10 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 86853-1-RR (same clone as 86853-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86853-1-RR (ABCB10 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86853-1-PBS in a different storage buffer formulation.

ABCB10 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 86853-1-PBS

Clone No.251920E7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

ABC transporter 10 protein, ABC-me protein, ABC-mitochondrial erythroid protein, ATP-binding cassette sub-family B member 10, mitochondrial, ATP-binding cassette transporter 10

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Product Information

86853-1-PBS targets ABCB10 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6203

  Product name: Recombinant human ABCB10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 452-738 aa of BC064930

  Sequence: YSELMKGLGAGGRLWELLEREPKLPFNEGVILNEKSFQGALEFKNVHFAYPARPEVPIFQDFSLSIPSGSVTALVGPSGSGKSTVLSLLLRLYDPASGTISLDGHDIRQLNPVWLRSKIGTVSQEPILFSCSIAENIAYGADDPSSVTAEEIQRVAEVANAVAFIRNFPQGFNTVVGEKGVLLSGGQKQRIAIARALLKNPKILLLDEATSALDAENEYLVQEALDRLMDGRTVLVIAHRLSTIKNANMVAVLDQGKITEYGKHEELLSKPNGIYRKLMNKQSFISA

• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 10
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC064930
• Gene Symbol: ABCB10
• Gene ID (NCBI): 23456
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NRK6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ABCB10 (also known as ABC-me or M-ABC2) is an inner-mitochondrial-membrane ATP-binding cassette transporter that forms homodimers with its nucleotide-binding domain facing the matrix. ABCB10 is now recognized as a multifunctional protein that couples mitochondrial export activity to cellular antioxidant defence. During red-cell development it stabilises the iron importer mitoferrin-1 (Mfrn1), thereby sustaining heme synthesis, whereas in cardiac and other non-erythroid tissues it attenuates oxidative damage by limiting mitochondrial ROS accumulation. PMID: 22884976