ABCB6 Polyclonal antibody

ABCB6 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14996-1-AP

Synonyms

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

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Validation Data Gallery

• 

  WB analysis of mouse brain using 14996-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 14996-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14996-1-AP (ABCB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14996-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14996-1-AP (ABCB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue
• Positive IHC detected in: human breast cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

14996-1-AP targets ABCB6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCB6 fusion protein Ag6982
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 6
• Calculated molecular weight: 94 kDa
• Observed molecular weight: 170 kDa
• GenBank accession number: BC000559
• Gene symbol: ABCB6
• Gene ID (NCBI): 10058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.