ABCB6 Polyclonal antibody

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  WB analysis using 51007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51007-1-AP (ABCB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 51007-1-AP

  Immunofluorescent analysis of MCF-7 cells using 51007-1-AP (ABCB6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ABCB6 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 51007-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

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Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 3 publications below

Product Information

51007-1-AP targets ABCB6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0461

  Product name: Recombinant human ABCB6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 57-267 aa of BC000559

  Sequence: AGADSLSWGAGPRISPYVLQLLLATLQAALPLAGLAGRVGTARGAPLPSYLLLASVLESLAGACGLWLLVVERSQARQRLAMGIWIKFRHSPGLLLLWTVAFAAENLALVSWNSPQWWWARADLGQQVQFSLWVLRYVVSGGLFVLGLWAPGLRPQSYTLQVHEEDQDVERSQVRSAAQQSTWRDFGRKLRLLSGYLWPRGSPALQLVVLI

• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 6
• Calculated Molecular Weight: 94 kDa
• Observed Molecular Weight: 70-79 kDa
• GenBank Accession Number: BC000559
• Gene Symbol: ABCB6
• Gene ID (NCBI): 10058
• RRID: AB_2220830
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NP58
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	J Invest Dermatol; Mutations in ABCB6 Cause Dyschromatosis Universalis Hereditaria.; Authors - Zhang Caie C
human	IHC	Cell Commun Signal; The ferroptosis and iron-metabolism signature robustly predicts clinical diagnosis, prognosis and immune microenvironment for hepatocellular carcinoma.; Authors - Bufu Tang
human	WB,IHC	J Biochem Mol Toxicol; SALL4/ABCB6 Axis Suppresses Ferroptosis in Colon Cancer by Mediating Mitophagy; Authors - Leilei Yang
human	WB	PLoS One; Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells.; Authors - Takafumi Nakano
human	WB	Free Radic Biol Med; Targeting ABCB6 with nitidine chloride inhibits PI3K/AKT signaling pathway to promote ferroptosis in multiple myeloma; Authors - Zhao Yin