ABCB6 Polyclonal antibody

ABCB6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 51007-1-AP

Synonyms

ABC, ABC14, ABCB6, EST45597, Mt ABC transporter 3, MTABC3, P glycoprotein related protein, PRP, umat

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Validation Data Gallery

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  WB analysis using 51007-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51007-1-AP (ABCB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 51007-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 51007-1-AP (ABCB6 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of MCF-7 using 51007-1-AP

  Immunofluorescent analysis of MCF-7 cells using 51007-1-AP (ABCB6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, U-251 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

51007-1-AP targets ABCB6 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCB6 fusion protein Ag0461
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 6
• Calculated molecular weight: 94 kDa
• Observed molecular weight: 80-84 kDa
• GenBank accession number: BC000559
• Gene symbol: ABCB6
• Gene ID (NCBI): 10058
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	WB	PLoS One; Hepatitis C virus core protein triggers abnormal porphyrin metabolism in human hepatocellular carcinoma cells.; Authors - Takafumi Nakano
human	IHC	Cell Commun Signal; The ferroptosis and iron-metabolism signature robustly predicts clinical diagnosis, prognosis and immune microenvironment for hepatocellular carcinoma.; Authors - Bufu Tang
human	WB,IHC	J Invest Dermatol; Mutations in ABCB6 Cause Dyschromatosis Universalis Hereditaria.; Authors - Zhang Caie C