ABCB8 Polyclonal antibody

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• ABCB8 Polyclonal antibody (25867-1-AP)

ABCB8 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 25867-1-AP Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

ABCB 8, ATP Binding Cassette Subfamily B Member 8, ATP-binding cassette sub-family B member 8, mitochondrial, M ABC1, MABC1

• 

  WB analysis using 25867-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25867-1-AP (ABCB8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IP experiment of HepG2 using 25867-1-AP

  IP result of anti-ABCB8 (IP:25867-1-AP, 4ug; Detection:25867-1-AP 1:800) with HepG2 cells lysate 1400 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of rat heart using 25867-1-AP

  IP result of anti-ABCB8 (IP:25867-1-AP, 4ug; Detection:25867-1-AP 1:800) with rat heart tissue lysate 2240 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 25867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25867-1-AP (ABCB8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse heart using 25867-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25867-1-AP (ABCB8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: rat heart tissue
• Positive IP detected in: HepG2 cells, rat heart tissue
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25867-1-AP targets ABCB8 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15304

  Product name: Recombinant human ABCB8 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 329-587 aa of BC141814

  Sequence: AFAMEQREEERYGAELEACRCRAEELGRGIALFQGLSNIAFNCMVLGTLFIGGSLVAGQQLTGGDLMSFLVASQTVQRSMANLSVLFGQVVRGLSAGARVFEYMALNPCIPLSGGCCVPKEQLRGSVTFQNVCFSYPCRPGFEVLKDFTLTLPPGKIVALVGQSGGGKTTVASLLERFYDPTAGVVMLDGRDLRTLDPSWLRGQVVGFISQEPVLFGTTIMENIRFGKLEASDEEVYTAAREANAHEFITSFPEGYNTV

• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 8
• Calculated Molecular Weight: 735 aa, 80 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC141814
• Gene Symbol: ABCB8
• Gene ID (NCBI): 11194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NUT2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCB8 is a mitochondrial inner membrane protein and a member of subfamily B of the ABC transporter superfamily. It contains two transmembrane domains (TMDs) and two nucleotide-binding domains (NBDs) and transports substrates in an ATP-dependent manner. This protein is a core regulator of mitochondrial iron homeostasis and is responsible for transporting iron-sulfur cluster (Fe-S) precursors from the mitochondrial matrix to the cytosol, thereby maintaining cellular iron homeostasis. Gene knockout or loss of function leads to mitochondrial iron accumulation, reactive oxygen species (ROS) burst, respiratory chain dysfunction, and apoptosis. In addition, ABCB8 is closely associated with iron metabolism-related diseases such as cardiomyopathy and Friedreich's ataxia, and is an important molecule in studies of mitochondrial iron transport, oxidative stress, and tumor metabolism.