ABCB9 Polyclonal antibody

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  WB analysis of BGC-823 using 11066-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 11066-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 11066-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 11066-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11066-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 11066-1-AP

  IP result of anti-ABCB9 (IP:11066-1-AP, 4ug; Detection:11066-1-AP 1:500) with mouse thymus tissue lysate 4000 ug.

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  IHC staining of mouse testis using 11066-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11066-1-AP (ABCB9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11066-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11066-1-AP (ABCB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11066-1-AP

  Immunofluorescent analysis of HepG2 cells, using ABCB9 antibody 11066-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

ABCB9 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11066-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

TAPL, EC:7.4.2.6, ATP-binding cassette transporter 9, ATP-binding cassette sub-family B member 9, ABC-type oligopeptide transporter ABCB9

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Tested Applications

• Positive WB detected in: BGC-823 cells, HL-60 cells, human testis tissue, K-562 cells, mouse thymus tissue
• Positive IP detected in: mouse thymus tissue
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11066-1-AP targets ABCB9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCB9 fusion protein Ag1542
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 9
• Calculated Molecular Weight: 84 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC017348
• Gene Symbol: ABCB9
• Gene ID (NCBI): 23457
• RRID: AB_10898357
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NP78
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oncogene; Suppression of lysosome metabolism-meditated GARP/TGF-β1 complexes specifically depletes regulatory T cells to inhibit breast cancer metastasis; Authors - Jing Ma