ABCB9 Polyclonal antibody

ABCB9 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11066-1-AP

Synonyms

ABC transporter 9 protein, ABCB9, EST122234, hABCB9, KIAA1520, TAP like protein, TAPL

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Validation Data Gallery

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  WB analysis of BGC-823 using 11066-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 11066-1-AP (ABCB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse thymus using 11066-1-AP

  IP result of anti-ABCB9 (IP:11066-1-AP, 4ug; Detection:11066-1-AP 1:500) with mouse thymus tissue lysate 4000 ug.

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  IF Staining of HepG2 using 11066-1-AP

  Immunofluorescent analysis of HepG2 cells, using ABCB9 antibody 11066-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: BGC-823 cells, HL-60 cells, human testis tissue, K-562 cells, mouse thymus tissue
• Positive IP detected in: mouse thymus tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Product Information

11066-1-AP targets ABCB9 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCB9 fusion protein Ag1542
• Full Name: ATP-binding cassette, sub-family B (MDR/TAP), member 9
• Calculated molecular weight: 84 kDa
• Observed molecular weight: 72 kDa
• GenBank accession number: BC017348
• Gene symbol: ABCB9
• Gene ID (NCBI): 23457
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.