ABCC10 Polyclonal antibody

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  WB analysis using 30697-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30697-1-AP (ABCC10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 30697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30697-1-AP (ABCC10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 30697-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30697-1-AP (ABCC10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ABCC10 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 30697-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

MRP7

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30697-1-AP targets ABCC10 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33719

  Product name: Recombinant human ABCC10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1203-1492 aa of NM_001198934.1

  Sequence: SSFTQTEAMLVSVERLEEYTCDLPQEPQGQPLQLGTGWLTQGGVEFQDVVLAYRPGLPNALDGVTFCVQPGEKLGIVGRTGSGKSSLLLVLFRLLEPSSGRVLLDGVDTSQLELAQLRSQLAIIPQEPFLFSGTVRENLDPQGLHKDRALWQALKQCHLSEVITSMGGLDGELGEGGRSLSLGQRQLLCLARALLTDAKILCIDEATASVDQKTDQLLQQTICKRFANKTVLTIAHRLNTILNSDRVLVLQAGRVVELDSPATLRNQPHSLFQQLLQSSQQGVPASLGGP*

• Full Name: ATP-binding cassette, sub-family C (CFTR/MRP), member 10
• Calculated Molecular Weight: 161kd
• Observed Molecular Weight: ~190 kDa
• GenBank Accession Number: NM_001198934.1
• Gene Symbol: ABCC10
• Gene ID (NCBI): 89845
• RRID: AB_3086392
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q5T3U5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The ATP binding cassette sub-family C member 10 (ABCC10), also named MRP7, is a cellular transmembrane protein transporter and belongs to the ATP-binding cassette (ABC) family. MRP7 is a lipophilic anion pump responsible for the transport of glucuronide E(2)17betaG and can confer resistance to chemotherapy drugs (PMID: 16868766). Since it is glycosylated, the apparent molecular weight of ABCC10 could be variable, ranging from 161 kDa to 191 kDa (PMID: 23087055; PMID: 24103790; PMID: 31338999; PMID: 34631561).