ABCD1 Monoclonal antibody

• Home
• Products
• Primary Antibodies
• ABCD1 Monoclonal antibody (60153-1-Ig)

• Featured Product
• KD/KO Validated

ABCD1 Monoclonal Antibody for WB, IF/ICC, ELISA

Cat No. 60153-1-Ig

Clone No.1H8E5

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

1H8E5, ABCD 1, ALD, ATP binding cassette sub family D member 1, ATP-binding cassette sub-family D member 1

• 

  WB analysis of HeLa using 60153-1-Ig

  WB result of ABCD1 antibody (60153-1-Ig, 1:1000) with si-Control and si-ABCD1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 60153-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 60153-1-Ig (ABCD1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 60153-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ABCD1 antibody (60153-1-Ig, Clone: 1H8E5 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).DAPI (blue).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60153-1-Ig targets ABCD1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12757

  Product name: Recombinant human ABCD1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 358-745 aa of BC025358

  Sequence: SESDAEAVKKAALEKKEEELVSERTEAFTIARNLLTAAADAIERIMSSYKEVTELAGYTARVHEMFQVFEDVQRCHFKRPRELEDAQAGSGTIGRSGVRVEGPLKIRGQVVDVEQGIICENIPIVTPSGEVVVASLNIRVEEGMHLLITGPNGCGKSSLFRILGGLWPTYGGVLYKPPPQRMFYIPQRPYMSVGSLRDQVIYPDSVEDMQRKGYSEQDLEAILDVVHLHHILQREGGWEAMCDWKDVLSGGEKQRIGMARMFYHRPKYALLDECTSAVSIDVEGKIFQAAKDAGIALLSITHRPSLWKYHTHLLQFDGEGGWKFEKLDSAARLSLTEEKQRLEQQLAGIPKMQRRLQELCQILGEAVAPAHVPAPSPQGPGGLQGAST

• Full Name: ATP-binding cassette, sub-family D (ALD), member 1
• Calculated Molecular Weight: 745 aa, 83 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: BC025358
• Gene Symbol: ABCD1
• Gene ID (NCBI): 215
• RRID: AB_10640436
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P33897
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCD1 (also known as ALDP) is a member of the ATP-binding cassette (ABC) transporter superfamily which functions as transporter for a wide variety of substrates. It localizes to the peroxisomal membrane. The exact function is not clear so far. Various mutations of ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), an inherited neurodegenerative disease affecting the nervous system white matter and adrenal cortex.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		EMBO Rep; Salmonella Typhimurium effector SseI regulates host peroxisomal dynamics to acquire lysosomal cholesterol; Authors - Desh Raj