ABCD3 Monoclonal antibody

ABCD3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human and More (2)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

1G7G5

Reviews (1)

Cat no : 66697-1-Ig

Synonyms

ABC43, ABCD3, PMP70, PXMP1

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Validation Data Gallery

• 

  WB analysis using 66697-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66697-1-Ig (ABCD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, HT-29 cells, Caco-2 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

66697-1-Ig targets ABCD3 in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: ABCD3 fusion protein Ag24760
• Full Name: ATP-binding cassette, sub-family D (ALD), member 3
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC068509
• Gene Symbol: ABCD3
• Gene ID (NCBI): 5825
• RRID: AB_2882050
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
		Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Life Sci Alliance; Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectors; Authors - Nnejiuwa U Ibe
human	WB	Haematologica; Dimeric ferrochelatase bridges ABCB7 and ABCB10 homodimers in an architecturally defined molecular complex required for heme biosynthesis.; Authors - Nunziata Maio
human	WB	bioRxiv; The p97-UBXD8 complex maintains peroxisome abundance by suppressing pexophagy; Authors - Iris D Montes
mouse	WB	Chin J Nat Med; Activation of LONP1 by 84-B10 alleviates aristolochic acid nephropathy via re-establishing mitochondrial and peroxisomal homeostasis; Authors - Xinyue Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

PAULA (Verified Customer) (07-12-2023)

I tested this ABCD3 antibody at two dilutions on THP1 cells (20ug cell lysate) and I saw a nice band around 70 kDa, although some non-specific bands were also detected. Dilution 1:1000 showed similar results to 1:500 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000, 1:500
• Cell Tissue Type: THP1 cell line

ABCD3 Antibody Western Blot validation (1:1000, 1:500 dilution) in THP1 cell line (Cat no:66697-1-Ig)