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  • KD/KO Validated

ABCF1 Polyclonal antibody

ABCF1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13950-1-AP

Synonyms

ABC27, ABC50, ABCF1, ATP binding cassette 50



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, HeLa cells
Positive IP detected inK-562 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13950-1-AP targets ABCF1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCF1 fusion protein Ag5061
Full Name ATP-binding cassette, sub-family F (GCN20), member 1
Calculated molecular weight 92 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC034488
Gene symbol ABCF1
Gene ID (NCBI) 23
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ABCF1 antibody 13950-1-APDownload protocol
IF protocol for ABCF1 antibody 13950-1-APDownload protocol
IP protocol for ABCF1 antibody 13950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

Immunity

The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock.

Authors - Hitesh Arora
mouse,humanWB

Sci Adv

ATP-binding cassette protein ABCF1 couples transcription and genome surveillance in embryonic stem cells through low-complexity domain.

Authors - Eun-Bee Choi
  • KD Validated
humanWB,IP

Proteomics

Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma

Authors - Han Hong