ABCF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ABCF1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 13950-1-AP

Synonyms

ABC27, ABC50, ABCF1, ATP binding cassette 50

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Validation Data Gallery

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  WB analysis using 13950-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13950-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13950-1-AP (ABCF1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 13950-1-AP

  IP Result of anti-ABCF1 (IP:13950-1-AP, 4ug; Detection:13950-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IF Staining of HeLa using 13950-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 13950-1-AP (ABCF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, HeLa cells
• Positive IP detected in: K-562 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

13950-1-AP targets ABCF1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCF1 fusion protein Ag5061
• Full Name: ATP-binding cassette, sub-family F (GCN20), member 1
• Calculated molecular weight: 92 kDa
• Observed molecular weight: 110 kDa
• GenBank accession number: BC034488
• Gene symbol: ABCF1
• Gene ID (NCBI): 23
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	CoIP	Immunity; The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock.; Authors - Hitesh Arora
mouse,human	WB	Sci Adv; ATP-binding cassette protein ABCF1 couples transcription and genome surveillance in embryonic stem cells through low-complexity domain.; Authors - Eun-Bee Choi ; KD Validated
human	WB,IP	Proteomics; Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma; Authors - Han Hong