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  • KD/KO Validated

ABCF1 Polyclonal antibody

ABCF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13950-1-AP

Synonyms

TNF-alpha-stimulated ABC protein, ATP-binding cassette sub-family F member 1, ATP-binding cassette 50, ATP binding cassette 50, ABCF 1



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HepG2 cells
Positive IP detected inK-562 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13950-1-AP targets ABCF1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABCF1 fusion protein Ag5061
Full Name ATP-binding cassette, sub-family F (GCN20), member 1
Calculated Molecular Weight 92 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC034488
Gene Symbol ABCF1
Gene ID (NCBI) 23
RRIDAB_2242044
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABCF1, also known as ABC27, and ABC50, is a member of the superfamily of ATP-binding cassette (ABC) transporters and contains two ABC transporter domains. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). Unlike other members of the superfamily, ABCF1 lacks the transmembrane domains that are characteristic of most ABC transporters. ABCF1 may be regulated by tumor necrosis factor-alpha and play a role in the enhancement of protein synthesis and thus participate in the inflammatory processes(PMID: 9790762).

Protocols

Product Specific Protocols
WB protocol for ABCF1 antibody 13950-1-APDownload protocol
IF protocol for ABCF1 antibody 13950-1-APDownload protocol
IP protocol for ABCF1 antibody 13950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
CoIP

Immunity

The ATP-Binding Cassette Gene ABCF1 Functions as an E2 Ubiquitin-Conjugating Enzyme Controlling Macrophage Polarization to Dampen Lethal Septic Shock.

Authors - Hitesh Arora
mouse,humanWB

Sci Adv

ATP-binding cassette protein ABCF1 couples transcription and genome surveillance in embryonic stem cells through low-complexity domain.

Authors - Eun-Bee Choi
  • KD Validated
humanWB,IP

Proteomics

Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma

Authors - Han Hong