ABCF3 Polyclonal antibody

ABCF3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15984-1-AP

Synonyms

ATP-binding cassette sub-family F member 3

---

Validation Data Gallery

• 

  WB analysis using 15984-1-AP

  Mouse heart lysates were subjected to SDS PAGE followed by western blot with 15984-1-AP (ABCF3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 15984-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 15984-1-AP (ABCF3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse heart tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15984-1-AP targets ABCF3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABCF3 fusion protein Ag8787
• Full Name: ATP-binding cassette, sub-family F (GCN20), member 3
• Calculated Molecular Weight: 709 aa, 80 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC009253
• Gene Symbol: ABCF3
• Gene ID (NCBI): 55324
• RRID: AB_2878205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.