ABHD15 Polyclonal antibody, PBS Only

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• ABHD15 Polyclonal antibody, PBS Only (22526-1-PBS)

• 

  WB analysis of mouse lung using 22526-1-AP (same clone as 22526-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 22526-1-AP (ABHD15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22526-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Transfected HEK-293 using 22526-1-AP (same clone as 22526-1-PBS)

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22526-1-AP (ABHD15 Antibody) at dilution of 1:700 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22526-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

ABHD15 Polyclonal Antibody for WB, Indirect ELISA

Cat No. 22526-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

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Product Information

22526-1-PBS targets ABHD15 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18334

  Product name: Recombinant human ABHD15 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 125-468 aa of BC012476

  Sequence: EYLQLADDGLVALDWVVGPCVRGRRITSAGGLPAVLLVIPNAWGRLTRNVLGLCLLALERGYYPVIFHRRGHHGCPLVSPRLQPFGDPSDLKEAVTYIRFRHPAAPLFAVSEGSGSALLLSYLGECGSSSYVTGAACISPVLRCREWFEAGLPWPYERGFLLYQKIALSRYATALEDTVDTSRLFRSRSLREFEEALFCHTKSFPISWDAYWDRNDPLRDVDEAAVPVLCICSADDPVCGPPDHTLTTELFHSNPYFFLLLSRHGGHCGFLRQEPLPAWSHEVILESFRALTEFFRTEERIKGLSRHRASFLGGRRRGGALQRREVSSSSNLEEIFNWKRSYTR

• Full Name: abhydrolase domain containing 15
• Calculated Molecular Weight: 468 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC012476
• Gene Symbol: ABHD15
• Gene ID (NCBI): 116236
• RRID: AB_2879117
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UXT9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ABHD15 belongs to the α/β-hydrolase superfamily which consists of various lipases, esterases, and proteases that share an α/β fold as a common structural feature. ABHD15 is highly expressed in adipose tissues and is required for the anti-lipolytic action of INS in white adipose tissue (WAT). ABHD15 expression is decreased in humans with obesity and diabetes compared to humans with obesity and normal glucose tolerance, identifying ABHD15 as a potential therapeutic target to mitigate INS resistance (PMID: 29768196).