ABHD17A Polyclonal antibody

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  WB analysis using 32439-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32439-1-AP (ABHD17A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ABHD17A Polyclonal Antibody for WB, ELISA

Cat No. 32439-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FAM108A1, Abhydrolase domain-containing protein 17A, Alpha/beta hydrolase domain-containing protein 17A, C19orf27, EC:3.1.2.22

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32439-1-AP targets ABHD17A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD17A fusion protein Ag36531
• Full Name: family with sequence similarity 108, member A1
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000158
• Gene Symbol: ABHD17A
• Gene ID (NCBI): 81926
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q96GS6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha/beta hydrolase domain-containing protein 17A (ABHD17A), also known as FAM108A, is a membrane-localized thioesterase, which regulates H- and N-RAS39, also catalyzes the depalmitoylation of CNAβ1 causing it to redistribute from the PM to the cytosol and the Golgi (PMID: 34663815). ABHD17a is a major acyl protein thioesterase that site-specifically deacylates the STREX domain of the BK channel to control channel activity (PMID: 32913120). Western blot analysis detected ABHD17A at an apparent molecular mass of 34 kDa (PMID: 27307232), and the 70 kDa-band could be the dimer.