ABHD5 Polyclonal antibody

ABHD5 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12201-1-AP

Synonyms

ABHD5, CDS, CGI58, IECN2, NCIE2



Tested Applications

Positive WB detected inJurkat cells, human brain tissue, human cerebellum tissue, mouse heart tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman skeletal muscle tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12201-1-AP targets ABHD5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, swine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ABHD5 fusion protein Ag2840
Full Name abhydrolase domain containing 5
Calculated molecular weight 349 aa, 39 kDa
Observed molecular weight 39 kDa
GenBank accession numberBC021958
Gene symbol ABHD5
Gene ID (NCBI) 51099
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

Protocols

Product Specific Protocols
WB protocol for ABHD5 antibody 12201-1-APDownload protocol
IHC protocol for ABHD5 antibody 12201-1-APDownload protocol
IF protocol for ABHD5 antibody 12201-1-APDownload protocol
IP protocol for ABHD5 antibody 12201-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
swineWB

Int J Obes (Lond)

Maternal obesity aggravates the abnormality of porcine placenta by increasing N6-methyladenosine.

Authors - Tongxing Song
mouseIP

Nat Metab

Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes.

Authors - Alexander Yang
mouseWB

PLoS One

Regulation of G0/G1 Switch Gene 2 (G0S2) Protein Ubiquitination and Stability by Triglyceride Accumulation and ATGL Interaction.

Authors - Bradlee L Heckmann
humanWB

J Lipid Res

CDP-diacylglycerol synthases regulate the growth of lipid droplets and adipocyte development.

Authors - Yanfei Qi
humanWB

Am J Physiol Endocrinol Metab

Regulation of FSP27 protein stability by AMPK and HSC70.

Authors - Xiaodong Zhang
mouseWB

J Biol Chem

Pleiotropic Effects of Cavin-1 Deficiency on Lipid Metabolism.

Authors - Shi-Ying Ding