ABHD5 Polyclonal antibody

• Featured Product
• KD/KO Validated

ABHD5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

28

Cat no : 12201-1-AP

Synonyms

IECN2, EC:2.3.1.51, CGI58, CDS, Abhydrolase domain-containing protein 5

---

Validation Data Gallery

• 

  WB analysis using 12201-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12201-1-AP (ABHD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12201-1-AP

  IP result of anti-ABHD5 (IP:12201-1-AP, 4ug; Detection:12201-1-AP 1:500) with Jurkat cells lysate 4000ug.

• 

  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12201-1-AP (ABHD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12201-1-AP (ABHD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 12201-1-AP (ABHD5 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12201-1-AP (ABHD5 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human liver using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 12201-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 12201-1-AP (ABHD5 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of MCF-7 using 12201-1-AP

  Immunofluorescent analysis of MCF-7 cells, using ABHD5 antibody 12201-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: A431 cells, A549 cells, HCT 116 cells, HeLa cells, HepG2 cells, LNCaP cells, NIH/3T3 cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon tissue, human kidney tissue, human liver tissue, human skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 25 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 2 publications below

Product Information

12201-1-AP targets ABHD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ABHD5 fusion protein Ag2840
• Full Name: abhydrolase domain containing 5
• Calculated Molecular Weight: 349 aa, 39 kDa
• Observed Molecular Weight: 39 kDa
• GenBank Accession Number: BC021958
• Gene Symbol: ABHD5
• Gene ID (NCBI): 51099
• RRID: AB_2220710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).

Publications

Species	Application	Title
		Nat Commun; PNPLA3 is a triglyceride lipase that mobilizes polyunsaturated fatty acids to facilitate hepatic secretion of large-sized very low-density lipoprotein; Authors - Scott M Johnson
mouse	WB	Nat Commun; O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.; Authors - Yunfan Yang
mouse	WB,CoIP	Cell Death Differ; Plin2-mediated lipid droplet mobilization accelerates exit from pluripotency by lipidomic remodeling and histone acetylation.; Authors - Yi Wu
human	WB	Proc Natl Acad Sci U S A; Spatial regulation of UBXD8 and p97/VCP controls ATGL-mediated lipid droplet turnover.; Authors - Olzmann James A JA
mouse	WB,IP,IF	Proc Natl Acad Sci U S A; Perilipins 2 and 3 lack a carboxy-terminal domain present in perilipin 1 involved in sequestering ABHD5 and suppressing basal lipolysis.; Authors - Satish Patel
human	WB	J Cell Biol; ORP5 localizes to ER-lipid droplet contacts and regulates the level of PI(4)P on lipid droplets.; Authors - Ximing Du