Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, A549 cells, HCT 116 cells, HepG2 cells, LNCaP cells, NIH/3T3 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon tissue, human kidney tissue, human liver tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12201-1-AP targets ABHD5 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2840 Product name: Recombinant human ABHD5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 6-349 aa of BC021958 Sequence: EEVDSADTGERSGWLTGWLPTWCPTSISHLKEAEEKMLKCVPCTYKKEPVRISNGNKIWTLKFSHNISNKTPLVLLHGFGGGLGLWALNFGDLCTNRPVYAFDLLGFGRSSRPRFDSDAEEVENQFVESIEEWRCALGLDKMILLGHNLGGFLAAAYSLKYPSRVNHLILVEPWGFPERPDLADQDRPIPVWIRALGAALTPFNPLAGLRIAGPFGLSLVQRLRPDFKRKYSSMFEDDTVTEYIYHCNVQTPSGETAFKNMTIPYGWAKRPMLQRIGKMHPDIPVSVIFGARSCIDGNSGTSIQSLRPHSYVKTIAILGAGHYVYADQPEEFNQKVKEICDTVD Predict reactive species |
| Full Name | abhydrolase domain containing 5 |
| Calculated Molecular Weight | 349 aa, 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC021958 |
| Gene Symbol | ABHD5 |
| Gene ID (NCBI) | 51099 |
| RRID | AB_2220710 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WTS1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ABHD5, also named as NCIE2 and CGI-58, belongs to the peptidase S33 family. ABHD4/ABHD5 subfamily. It is a Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. ABHD5 may regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. It is involved in keratinocyte differentiation. ABHD5 is an evolutionarily conserved protein that acts as a potent activator of Atgl. Abhd5 is expressed in several tissues that lack Plin , raising the possibility that this co-activator might interact with additional PAT proteins. Abhd5 and Mldp are highly colocalized on individual lipid droplets.(PMID:19064991) Defects in ABHD5 are the cause of Chanarin-Dorfman syndrome (CDS).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| IHC protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| IP protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| WB protocol for ABHD5 antibody 12201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ABHD5 monoclonal antibody (Cat# 12201-1-AP) has 37 citations spanning journals including Nature Metabolism, Nature Communications, PNAS, Journal of Biological Chemistry, Developmental Cell, Journal of Clinical Investigation, and Journal of Lipid Research. Research fields encompass lipid droplet biology, lipolysis regulation, obesity, hepatic steatosis, cancer cell metabolism, adipocyte differentiation, and AMPK/mTOR signaling pathways.
| Species | Application | Title |
|---|---|---|
Nat Metab Dynamic interactions of ABHD5 with PNPLA3 regulate triacylglycerol metabolism in brown adipocytes. | ||
Nat Metab Peroxisomal β-oxidation acts as a sensor for intracellular fatty acids and regulates lipolysis. | ||
Nat Commun PNPLA3 is a triglyceride lipase that mobilizes polyunsaturated fatty acids to facilitate hepatic secretion of large-sized very low-density lipoprotein | ||
Nat Commun O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. | ||
Metabolism Prolyl isomerase Pin1 interacts with adipose triglyceride lipase and negatively controls both its expression and lipolysis. | ||
J Clin Invest Identification of lysosomal lipolysis as an essential noncanonical mediator of adipocyte fasting and cold-induced lipolysis |
