Tested Applications
| Positive WB detected in | NIH/3T3 cells, mouse brain tissue, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27981-1-AP targets ABLIM3 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27578 Product name: Recombinant human ABLIM3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 336-536 aa of BC001665 Sequence: YYRSGDLSTATKSKTSEDISQTSKYSPIYSPDPYYASESEYWTYHGSPKVPRARRFSSGGEEDDFDRSMHKLQSGIGRLILKEEMKARSSSYADPWTPPRSSTSSREALHTAGYEMSLNGSPRSHYLADSDPLISKSASLPAYRRNGLHRTPSADLFHYDSMNAVNWGMREYKIYPYELLLVTTRGRNRLPKDVDRTRLEG Predict reactive species |
| Full Name | actin binding LIM protein family, member 3 |
| Calculated Molecular Weight | 78 kDa |
| Observed Molecular Weight | 78 kDa |
| GenBank Accession Number | BC001665 |
| Gene Symbol | ABLIM3 |
| Gene ID (NCBI) | 22885 |
| RRID | AB_2881029 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O94929 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ABLIM3 antibody 27981-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ABLIM3 Polyclonal antibody (Cat# 27981-1-AP) has a total of 1 citation. It was cited in Molecular Biology of the Cell (2021), in a study investigating the spatial proximity of proteins surrounding zyxin under force-bearing conditions, reflecting its application in cell biology and mechanobiology research via Western blot.
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Spatial proximity of proteins surrounding zyxin under force-bearing conditions. |
Reviews
What customers say
The product received one 5-star review from a researcher who used it on MDCK GII cells for Western Blot at a 1:1k dilution. The reviewer reported that the antibody produced a prominent band with little background, indicating strong specificity and clean signal in their application. Overall, the feedback is positive, highlighting reliable performance and good signal-to-noise ratio in Western Blot experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Joleen (Verified Customer) (05-29-2019) | It works on western blot with a prominent band and little background.
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