ACAD9 Recombinant monoclonal antibody

• 

  WB analysis using 84202-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84202-1-RR (ACAD9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

ACAD9 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84202-1-RR

Clone No.241015B3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

241015B3, ACAD 9, ACAD-9, Acyl-CoA dehydrogenase family member 9, Complex I assembly factor ACAD9, mitochondrial

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Tested Applications

• Positive WB detected in: HEK-293T cells, MCF-7 cells, mouse heart tissue, mouse brain tissue, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84202-1-RR targets ACAD9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8414

  Product name: Recombinant human ACAD9 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 285-621 aa of BC007970

  Sequence: ILGEVGDGFKVAMNILNSGRFSMGSVVAGLLKRLIEMTAEYACTRKQFNKRLSEFGLIQEKFALMAQKAYVMESMTYLTAGMLDQPGFPDCSIEAAMVKVFSSEAAWQCVSEALQILGGLGYTRDYPYERILRDTRILLIFEGTNEILRMYIALTGLQHAGRILTTRIHELKQAKVSTVMDTVGRRLRDSLGRTVDLGLTGNHGVVHPSLADSANKFEENTYCFGRTVETLLLRFGKTIMEEQLVLKRVANILINLYGMTAVLSRASRSIRIGLRNHDHEVLLANTFCVEAYLQNLFSLSQLDKYAPENLDEQIKKVSQQILEKRAYICAHPLDRTC

• Full Name: acyl-Coenzyme A dehydrogenase family, member 9
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC007970
• Gene Symbol: ACAD9
• Gene ID (NCBI): 28976
• RRID: AB_3671758
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9H845
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Acyl-CoA dehydrogenases (ACADs) are a family of mitochondrial enzymes catalyzing the initial rate-limiting step in the β-oxidation of fatty acyl-CoA. ACAD9 belongs to the group of ACADs. The deduced 621-amino acid protein has a calculated molecular mass of 68.8 kD. It has an N-terminal leader sequence, 2 conserved motifs shared by all ACAD family members, and a potential N-glycosylation site(PMID:12359260). Defects in ACAD9 are a cause of acyl-CoA dehydrogenase family member type 9 deficiency (ACAD9 deficiency).