ACADL Polyclonal antibody

ACADL Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17442-1-AP

Synonyms

ACAD4, ACADL, LCAD



Tested Applications

Positive WB detected inmouse kidney tissue, HepG2 cells, rat kidney tissue, HeLa cells, HEK-293 cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

17442-1-AP targets ACADL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ACADL fusion protein Ag11530
Full Name acyl-Coenzyme A dehydrogenase, long chain
Calculated molecular weight 430 aa, 48 kDa
Observed molecular weight 45-48 kDa
GenBank accession numberBC039063
Gene symbol ACADL
Gene ID (NCBI) 33
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065).Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).

Protocols

Product Specific Protocols
WB protocol for ACADL antibody 17442-1-APDownload protocol
IHC protocol for ACADL antibody 17442-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients.

Authors - Ya-Kun Zhang