ACADL-Specific Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85379-1-RR (same clone as 85379-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85379-1-RR (ACADL-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85379-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 85379-1-RR (same clone as 85379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85379-1-RR (ACADL-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat liver using 85379-1-RR (same clone as 85379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 85379-1-RR (ACADL-Specific antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85379-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 85379-1-RR (same clone as 85379-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85379-1-RR (ACADL-Specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85379-1-PBS in a different storage buffer formulation.

ACADL-Specific Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 85379-1-PBS

Clone No.242413B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

ACADL, 242413B10, ACAD4, EC:1.3.8.8, LCAD

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Product Information

85379-1-PBS targets ACADL-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: acyl-Coenzyme A dehydrogenase, long chain
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-48 kDa
• GenBank Accession Number: NM_001608
• Gene Symbol: ACADL
• Gene ID (NCBI): 33
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P28330
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

ACADL(Long-chain specific acyl-CoA dehydrogenase, mitochondrial) is also named as LCAD and belongs to the acyl-CoA dehydrogenase family.It catalyzes the first reaction of the mitochondrial β-oxidation of fatty acids and is synthesized in the cytosol as a precursor that is larger than its mature from. The size difference between the precursor and mature rat LCAD has been estimated to be 3 kDa on the basis of SDS-PAGE(PMID:1774065). Defects in ACADL are a cause of acyl-CoA dehydrogenase very long-chain deficiency (ACADVLD).This antibody is specific to ACADL.