ACAP2 Polyclonal antibody

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  WB analysis of NIH/3T3 using 14029-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 14029-1-AP (ACAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14029-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14029-1-AP (ACAP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14029-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14029-1-AP (ACAP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of NIH/3T3 using 14029-1-AP

  IP result of anti-ACAP2 (IP:14029-1-AP, 4ug; Detection:14029-1-AP 1:300) with NIH/3T3 cells lysate 3600ug.

• Featured Product
• KD/KO Validated

ACAP2 Polyclonal Antibody for WB, IP, ELISA

Cat No. 14029-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, ELISA

Synonyms

ACAP2, Centaurin beta 2, CENTB2, CNT B2, KIAA0041

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Jurkat cells
• Positive IP detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Product Information

14029-1-AP targets ACAP2 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ACAP2 fusion protein Ag5190
• Full Name: ArfGAP with coiled-coil, ankyrin repeat and PH domains 2
• Calculated Molecular Weight: 778 aa, 88 kDa
• Observed Molecular Weight: 88 kDa
• GenBank Accession Number: BC060767
• Gene Symbol: ACAP2
• Gene ID (NCBI): 23527
• RRID: AB_2219956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15057
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
canine	WB,IF	J Biol Chem; Aberrant endocytosis leads to the loss of normal mitotic spindle orientation during epithelial glandular morphogenesis.; Authors - James W Clancy
mouse	WB	Cell Death Dis; Rab35 and glucocorticoids regulate APP and BACE1 trafficking to modulate Aβ production.; Authors - Viktoriya Zhuravleva ; KD Validated